Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HNRNPR / hnRNP R Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G118792-100
Catalog Number: LS-G118792
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human hnRNP R(HNRNPR) (NM_001102397), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HnRNP-R | HNRNPR | HNRPR | HnRNP R
- Origin Species
- Human
- NCBI GenBank
- NM_005826
- SwissProt
- O43390
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 59.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKTYRQREKQ GSKVQESTKG PDEAKIKALL ERTGYTLDVT TGQRKYGGPP PDSVYSGVQP61GIGTEVFVGK IPRDLYEDEL VPLFEKAGPI WDLRLMMDPL SGQNRGYAFI TFCGKEAAQE121AVKLCDSYEI RPGKHLGVCI SVANNRLFVG SIPKNKTKEN ILEEFSKVTE GLVDVILYHQ181PDDKKKNRGF CFLEYEDHKS AAQARRRLMS GKVKVWGNVV TVEWADPVEE PDPEVMAKVK241VLFVRNLATT VTEEILEKSF SEFGKLERVK KLKDYAFVHF EDRGAAVKAM DEMNGKEIEG301EEIEIVLAKP PDKKRKERQA ARQASRSTAY EDYYYHPPPR MPPPIRGRGR GGGRGGYGYP361PDYYGYEDYY DDYYGYDYHD YRGGYEDPYY GYDDGYAVRG RGGGRGGRGA PPPPRGRGAP421PPRGRAGYSQ RGAPLGPPRG SRGGRGGPAQ QQRGRGSRGS RGNRGGNVGG KRKADGYNQP481DSKRRQTNNQ QNWGSQPIAQ QPLQQGGDYS GNYGYNNDNQ EFYQDTYGQQ WKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
532 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.