Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HnRNPLL / HNRPLL Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G126070-100
Catalog Number: LS-G126070
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HNRPLL(HNRNPLL) (NM_001142650), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HnRNPLL | HNRPLL | SRRF | Stromal RNA regulating factor | Stromal RNA-regulating factor
- Origin Species
- Human
- NCBI GenBank
- NM_138394
- SwissProt
- Q8WVV9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 59.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLARETYEED REYESQAKRL KTEEGEIDYS AEEGENRREA TPRGGGDGGG GGRSFSQPEA61GGSHHKVSVS PVVHVRGLCE SVVEADLVEA LEKFGTICYV MMMPFKRQAL VEFENIDSAK121ECVTFAADEP VYIAGQQAFF NYSTSKRITR PGNTDDPSGG NKVLLLSIQN PLYPITVDVL181YTVCNPVGKV QRIVIFKRNG IQAMVEFESV LCAQKAKAAL NGADIYAGCC TLKIEYARPT241RLNVIRNDND SWDYTKPYLG RRDRGKGRQR QAILGEHPSS FRHDGYGSHG PLLPLPSRYR301MGSRDTPELV AYPLPQASSS YMHGGNPSGS VVMVSGLHQL KMNCSRVFNL FCLYGNIEKV361KFMKTIPGTA LVEMGDEYAV ERAVTHLNNV KLFGKRLNVC VSKQHSVVPS QIFELEDGTS421SYKDFAMSKN NRFTSAGQAS KNIIQPPSCV LHYYNVPLCV TEETFTKLCN DHEVLTFIKY481KVFDAKPSAK TLSGLLEWEC KTDAVEALTA LNHYQIRVPN GSNPYTLKLC FSTSSHLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
537 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.