Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HnRNPLL / HNRPLL Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G107366-100
Catalog Number: LS-G107366
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HNRPLL(HNRNPLL) (NM_138394), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HnRNPLL | HNRPLL | SRRF | Stromal RNA regulating factor | Stromal RNA-regulating factor
- Origin Species
- Human
- NCBI GenBank
- NM_138394
- SwissProt
- Q8WVV9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 59.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSSSSSSPRE TYEEDREYES QAKRLKTEEG EIDYSAEEGE NRREATPRGG GDGGGGGRSF61SQPEAGGSHH KVSVSPVVHV RGLCESVVEA DLVEALEKFG TICYVMMMPF KRQALVEFEN121IDSAKECVTF AADEPVYIAG QQAFFNYSTS KRITRPGNTD DPSGGNKVLL LSIQNPLYPI181TVDVLYTVCN PVGKVQRIVI FKRNGIQAMV EFESVLCAQK AKAALNGADI YAGCCTLKIE241YARPTRLNVI RNDNDSWDYT KPYLGRRDRG KGRQRQAILG EHPSSFRHDG YGSHGPLLPL301PSRYRMGSRD TPELVAYPLP QASSSYMHGG NPSGSVVMVS GLHQLKMNCS RVFNLFCLYG361NIEKVKFMKT IPGTALVEMG DEYAVERAVT HLNNVKLFGK RLNVCVSKQH SVVPSQIFEL421EDGTSSYKDF AMSKNNRFTS AGQASKNIIQ PPSCVLHYYN VPLCVTEETF TKLCNDHEVL481TFIKYKVFDA KPSAKTLSGL LEWECKTDAV EALTALNHYQ IRVPNGSNPY TLKLCFSTSS541HLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
542 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.