Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HNRNPL / hnRNP L Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G122348-100
Catalog Number: LS-G122348
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human hnRNP L(HNRNPL) (NM_001005335), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HnRNP L | HnRNP-L | HNRNPL | HNRPL | P/OKcl.14
- Origin Species
- Human
- NCBI GenBank
- NM_001533
- SwissProt
- P14866
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 60.23 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPKKRQALVE FEDVLGACNA VNYAADNQIY IAGHPAFVNY STSQKISRPG DSDDSRSVNS61VLLFTILNPI YSITTDVLYT ICNPCGPVQR IVIFRKNGVQ AMVEFDSVQS AQRAKASLNG121ADIYSGCCTL KIEYAKPTRL NVFKNDQDTW DYTNPNLSGQ GDPGSNPNKR QRQPPLLGDH181PAEYGGPHGG YHSHYHDEGY GPPPPHYEGR RMGPPVGGHR RGPSRYGPQY GHPPPPPPPP241EYGPHADSPV LMVYGLDQSK MNCDRVFNVF CLYGNVEKVK FMKSKPGAAM VEMADGYAVD301RAITHLNNNF MFGQKLNVCV SKQPAIMPGQ SYGLEDGSCS YKDFSESRNN RFSTPEQAAK361NRIQHPSNVL HFFNAPLEVT EENFFEICDE LGVKRPSSVK VFSGKSERSS SGLLEWESKS421DALETLGFLN HYQMKNPNGP YPYTLKLCFS TAQHASTRTRPLEQKLISEEDLAANDILDYKDDDDKV
456 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.