Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HNF4A / HNF4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G120829-100
Catalog Number: LS-G120829
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HNF 4 alpha(HNF4A) (NM_001030003), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Hepatic nuclear factor 4 alpha | HNF4 | HNF4a8 | NR2A21 | TCF | TCF-14 | TCF14 | MODY1 | Transcription factor 14 | Transcription factor HNF-4 | HNF-4-alpha | HNF4 alpha | HNF4A | HNF4a7 | HNF4a9 | HNF4alpha | HNF4alpha10/11/12 | MODY | NR2A1
- Origin Species
- Human
- NCBI GenBank
- NM_000457
- SwissProt
- P41235
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 48.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVSVNAPLGA PVESSYDTSP SEGTNLNAPN SLGVSALCAI CGDRATGKHY GASSCDGCKG61FFRRSVRKNH MYSCRFSRQC VVDKDKRNQC RYCRLKKCFR AGMKKEAVQN ERDRISTRRS121SYEDSSLPSI NALLQAEVLS RQITSPVSGI NGDIRAKKIA SIADVCESMK EQLLVLVEWA181KYIPAFCELP LDDQVALLRA HAGEHLLLGA TKRSMVFKDV LLLGNDYIVP RHCPELAEMS241RVSIRILDEL VLPFQELQID DNEYAYLKAI IFFDPDAKGL SDPGKIKRLR SQVQVSLEDY301INDRQYDSRG RFGELLLLLP TLQSITWQMI EQIQFIKLFG MAKIDNLLQE MLLGGSPSDA361PHAHHPLHPH LMQEHMGTNV IVANTMPTHL SNGQMSTPET PQPSPPGGSG SEPYKLLPGA421VATIVKPLSA IPQPTITKQE VITRTRPLEQKLISEEDLAANDILDYKDDDDKV
442 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.