Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HMMR / CD168 / RHAMM Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G114394-100
Catalog Number: LS-G114394
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CD168(HMMR) (NM_012485), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CD168 | CD168 antigen | HMMR | RHAMM | IHABP
- Origin Species
- Human
- NCBI GenBank
- NM_012484
- SwissProt
- O75330
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 82.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSFPKAPLKR FNDPSGCAPS PGAYDVKTLE VLKGPVSFQK SQRFKQQKES KQNLNVDKDT61TLPASARKVK SSESKIRVLL QERGAQDRRI QDLETELEKM EARLNAALRE KTSLSANNAT121LEKQLIELTR TNELLKSKFS ENGNQKNLRI LSLELMKLRN KRETKMRGMM AKQEGMEMKL181QVTQRSLEES QGKIAQLEGK LVSIEKEKID EKSETEKLLE YIEEISCASD QVEKYKLDIA241QLEENLKEKN DEILSLKQSL EENIVILSKQ VEDLNVKCQL LEKEKEDHVN RNREHNENLN301AEMQNLKQKF ILEQQEREKL QQKELQIDSL LQQEKELSSS LHQKLCSFQE EMAKEKNLFE361EELKQTLDEL DKLQQKEEQA ERLVKQLEEE AKSRAEELKL LEEKLKGKEA ELEKSSAAHT421QATLLLQEKY DSMVQSLEDV TAQFESYKAL TASEIEDLKL ENSSLQEKAA KAGKNAEDVQ481HQILATESSN QEYVRMLLDL QTKSALKETE IKEITVSFLQ KITDLQNQLK QQEEDFRKQL541EDEEGRKAEK ENTTAELTEE INKWRLLYEE LYNKTKPFQL QLDAFEVEKQ ALLNEHGAAQ601EQLNKIRDSY AKLLGHQNLK QKIKHVVKLK DENSQLKSEV SKLRCQLAKK KQSETKLQEE661LNKVLGIKHF DPSKAFHHES KENFALKTPL KEGNTNCYRA PMECQESWKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
709 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.