Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HMGXB3 / SMF Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G127080-100
Catalog Number: LS-G127080
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HMGXB3 (NM_014983), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HMG box-containing protein 3 | HMGXB3 | HMG box domain containing 3 | KIAA0194 | SMF | Protein SMF | HMGX3
- Origin Species
- Human
- NCBI GenBank
- D83778
- SwissProt
- Q12766
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 141 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDASYDGTEV TVVMEEIEEA YCYTSPGPPK KKKKYKIHGE KTKKPRSAYL LYYYDIYLKV61QQELPHLPQS EINKKISESW RLLSVAERSY YLEKAKLEKE GLDPNSKLSA LTAVVPDIPG121FRKILPRSDY IIIPKSSLQE DRSCPQLELC VAQNQMSPKG PPLVSNTAPE TVPSHAGMAE181QCLAVEALAE EVGALTQSGA VQEIATSEIL SQDVLLEDAS LEVGESHQPY QTSLVIEETL241VNGSPDLPTG SLAVPHPQVG ESISVVTVMR DSSESSSSAP ATQFIMLPLP AYSVVENPTS301IKLTTTYTRR GHGTCTSPGC SFTYVTRHKP PKCPTCGNFL GGKWIPKEKP AKVKVELASG361VSSKGSVVKR NQQPVTTEQN SSKENASKLT LENSEAVSQL LNVAPPREVG EESEWEEVII421SDAHVLVKEA PGNCGTAVTK TPVVKSGVQP EVTLGTTDND SPGADVPTPS EGTSTSSPLP481APKKPTGVDL LTPGSRAPEL KGRARGKPSL LAAARPMRAI LPAPVNVGRG SSMGLPRARQ541AFSLSDKTPS VRTCGLKPST LKQLGQPIQQ PSGPGEVKLP SGPSNRTSQV KVVEVKPDMF601PPYKYSCTVT LDLGLATSRG RGKCKNPSCS YVYTNRHKPR ICPSCGVNLA KDRTEKTTKA661IEVSSPLPDV LNATEPLSTA QREIQRQSTL QLLRKVLQIP ENESELAEVF ALIHELNSSR721LILSNVSEET VTIEQTSWSN YYESPSTQCL LCSSPLFKGG QNSLAGPQEC WLLTASRLQT781VTAQVKMCLN PHCLALHSFI DIYTGLFNVG NKLLVSLDLL FAIRNQIKLG EDPRVSINVV841LKSVQEQTEK TLTSEELSQL QELLCNGYWA FECLTVRDYN DMICGICGVA PKVEMAQRSE901ENVLALKSVE FTWPEFLGSN EVNVEDFWAT METEVIEQVA FPASIPITKF DASVIAPFFP961PLMRGAVVVN TEKDKNLDVQ PVPGSGSALV RLLQEGTCKL DEIGSYSEEK LQHLLRQCGI1021PFGAEDSKDQ LCFSLLALYE SVQNGARAIR PPRHFTGGKI YKVCPHQVVC GSKYLVRGES1081ARDHVDLLAS SRHWPPVYVV DMATSVALCA DLCYPELTNQ MWGRNQGCFS SPTEPPVSVS1141CPELLDQHYT VDMTETEHSI QHPVTKTATR RIVHAGLQPN PGDPSAGHHS LALCPELAPY1201ATILASIVDS KPNGVRQRPI AFDNATHYYL YNRLMDFLTS REIVNRQIHD IVQSCQPGEV1261VIRDTLYRLG VAQIKTETEE EGEEEEVAAV AETRTRPLEQKLISEEDLAANDILDYKDDDDKV
1292 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.