Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HMG2L1 / HMGXB4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G114071-100
Catalog Number: LS-G114071
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HMG2L1(HMGXB4) (NM_014250), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HMGBCG | HMGXB4 | HMG2L1 | HMG box domain containing 4 | HMG box-containing protein 4 | Protein HMGBCG | THC211630
- Origin Species
- Human
- NCBI GenBank
- AL079310
- SwissProt
- Q9UGU5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 53 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDLLKAITSP LAAGSKPSKK TGEKSSGSSS HSESKKEHHR KKVSGSSGEL PLEDGGSHKS61KKMKPLYVNT ETLTLREPDG LKMKLILSPK EKGSSSVDEE SFQYPSQQAT VKKSSKKSAR121DEQGALLLGH ELQSFLKTAR KKHKSSSDAH SSPGPEGCGS DASQFAESHS ANLDLSGLEP181ILVESDSSSG GELEAGELVI DDSYREIKKK KKSKKSKKKK DKEKHKEKRH SKSKRSLGLS241AVPVGEVTVT SGPPPSIPYA GAAAPPLPLP GLHTDGHSEK KKKKEEKDKE RERGEKPKKK301NMSAYQVFCK EYRVTIVADH PGIDFGELSK KLAEVWKQLP EKDKLIWKQK AQYLQHKQNK361AEATTVKRKA SSSEGSMKVK ASSVGVLSPQ KKSPPTTMLL PASPAKAPET EPIDVAAHLQ421LLGESLSLIG HRLQETEGMV AVSGSLSVLL DSIICALGPL ACLTTQLPEL NGCPKQVLSN481TLDNIAYIMP GLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
492 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.