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HMG20A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HMG20A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G111921-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HMG20A (NM_018200), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HMG20A | HMGXB1 | High-mobility group 20A | HMG box domain containing 1 | High mobility group 20A | HMG box-containing protein 20A | HMGX1
Origin Species
Human
NCBI GenBank
NM_018200
SwissProt
Q9NP66

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
40 kDa
Protein Type
Lysate
Amino Acid Sequence
1MENLMTSSTL PPLFADEDGS KESNDLATTG LNHPEVPYSS GATSSTNNPE FVEDLSQGQL
61LQSESSNAAE GNEQRHEDEQ RSKRGGWSKG RKRKKPLRDS NAPKSPLTGY VRFMNERREQ
121LRAKRPEVPF PEITRMLGNE WSKLPPEEKQ RYLDEADRDK ERYMKELEQY QKTEAYKVFS
181RKTQDRQKGK SHRQDAARQA THDHEKETEV KERSVFDIPI FTEEFLNHSK AREAELRQLR
241KSNMEFEERN AALQKHVESM RTAVEKLEVD VIQERSRNTV LQQHLETLRQ VLTSSFASMP
301LPGSGETPTV DTIDSYMNRL HSIILANPQD NENFIATVRE VVNRLDRTRT RPLEQKLISE
361EDLAANDILD YKDDDDKV

378 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.