Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HMCES / C3orf37 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G111291-100
Catalog Number: LS-G111291
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C3orf37(HMCES) (NM_020187), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C85376 | C3orf37 | DC12 | RIKEN cDNA 8430410A17 gene | UPF0361 protein C3orf37 | SRAPD1
- Origin Species
- Human
- NCBI GenBank
- NM_001006109
- SwissProt
- Q96FZ2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 40.4 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MCGRTSCHLP RDVLTRACAY QDRRGQQRLP EWRDPDKYCP SYNKSPQSNS PVLLSRLHFE61KDADSSERII APMRWGLVPS WFKESDPSKL QFNTTNCRSD TVMEKRSFKV PLGKGRRCVV121LADGFYEWQR CQGTNQRQPY FIYFPQIKTE KSGSIGAADS PENWEKVWDN WRLLTMAGIF181DCWEPPEGGD VLYSYTIITV DSCKGLSDIH HRMPAILDGE EAVSKWLDFG EVSTQEALKL241IHPTENITFH AVSSVVNNSR NNTPECLAPV DLVVKKELRA SGSSQRMLQW LATKSPKKED301SKTPQKEESD VPQWSSQFLQ KSPLPTKRGT AGLLEQWLKR EKEEEPVAKR PYSQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
354 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.