Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HM13 / IMP-1 / SPP Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G102494-100
Catalog Number: LS-G102494
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Signal Peptide Peptidase(HM13) (NM_178581), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DJ324O17.1 | HM13 | HIMP1 | IMP1 | Intramembrane protease 1 | H13 | MSTP086 | IMP-1 | IMPAS | IMPAS-1 | Presenilin-like protein 3 | PSL3 | Signal peptide peptidase | Signal peptide peptidase beta | SPP | PSENL3 | SPPL1
- Origin Species
- Human
- NCBI GenBank
- NM_030789
- SwissProt
- Q8TCT9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 46.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MDSALSDPHN GSAEAGGPTN STTRPPSTPE GIALAYGSLL LMALLPIFFG ALRSVRCARG61KNASDMPETI TSRDAARFPI IASCTLLGLY LFFKIFSQEY INLLLSMYFF VLGILALSHT121ISPFMNKFFP ASFPNRQYQL LFTQGSGENK EEIINYEFDT KDLVCLGLSS IVGVWYLLRK181HWIANNLFGL AFSLNGVELL HLNNVSTGCI LLGGLFIYDV FWVFGTNVMV TVAKSFEAPI241KLVFPQDLLE KGLEANNFAM LGLGDVVIPG IFIALLLRFD ISLKKNTHTY FYTSFAAYIF301GLGLTIFIMH IFKHAQPALL YLVPACIGFP VLVALAKGEV TEMFSYESSA EILPHTPRLT361HFPTVSGSPA SLADSMQQKL AGPRRRRPQN PSAIYEESNP KDPAAVTESK EGTEASASKG421LEKKEKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
426 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.