LSBio
HLA-DQA1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HLA-DQA1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all HLA-DQA1 antibodies →
$504.00each
Catalog Number: LS-G99656-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HLADQA1(HLA) (NM_002122), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DC-alpha | DQ-A1 | DC-1 alpha chain | HLA-DQA1 | Leukocyte antigen alpha chain | GSE | MHC class II DQA1 | HLA-DCA | HLA-DQA | MHC class II antigen | CELIAC1 | Leucocyte antigen DQA1 | MHC class II HLA-DQ-alpha-1 | MHC HLA-DQ alpha
NCBI GenBank
NM_002122
SwissProt
P01909

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
27.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MILNKALLLG ALALTTVMSP CGGEDIVADH VASCGVNLYQ FYGPSGQFTH EFDGDEQFYV
61DLEKKETAWR WPEFSKFGGF DPQGALRNMA VAKHNLNIMI KRYNSTAATN EVPEVTVFSK
121SPVTLGQPNT LICLDNIFPP VVNITWLSNG HAVTEGVSET SFLSKSDHSF FKISYLTFLP
181SADEIYDCKV EHWGLDQPLL KHWEPEIPAP MSELTETVVC ALGLSVGLVG IVVGTVFIIQ
241GLRSVGASRH QGPL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

254 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.