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HLA-DPB1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HLA-DPB1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G99658-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HLA (NM_002121), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Class II HLA beta chain | HLA-DP1B | HLA-DPB1 | MHC class II antigen DPbeta1 | MHC class II antigen DPB1 | MHC class II HLA-DRB1 | MHC HLA DPB1 | HLA-DPB | MHC class II HLA-DP-beta-1 | DPB1 | HLA DP14-beta chain | MHC class II HLA-DP-beta
NCBI GenBank
NM_002121
SwissProt
P04440

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
29.25 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVLQVSAAPR TVALTALLMV LLTSVVQGRA TPENYVHQLR QECYAFNGTQ RFLERYIYNR
61EEFVRFDSDV GEFRAVTELG RPDEDYWNSQ KDILEEERAV PDRMCRHNYE LDEAVTLQRR
121VQPRVNVSPS KKGPLQHHNL LVCHVTDFYP GSIQVRWFLN GQEETAGVVS TNLIRNGDWT
181FQILVMLEMT PQQGDVYTCQ VEHTSLDSPV TVEWKAQSDS ARSKTLTGAG GFVLGLIICG
241VGIFMHRRSK KVQRGSA
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

257 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.