Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HIWI2 / PIWIL4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106257-100
Catalog Number: LS-G106257
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PIWIL4 (NM_152431), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PIWI | PIWIL4 | Piwi-like 4 (Drosophila) | HIWI2 | MIWI2 | Piwi-like protein 4
- Origin Species
- Human
- NCBI GenBank
- BC031060
- SwissProt
- Q7Z3Z4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 96.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSGRARVKAR GIARSPSATE VGRIQASPLP RSVDLSNNEA SSSNGFLGTS RISTNDKYGI61SSGDAGSTFM ERGVKNKQDF MDLSICTREK LAHVRNCKTG SSGIPVKLVT NLFNLDFPQD121WQLYQYHVTY IPDLASRRLR IALLYSHSEL SNKAKAFDGA ILFLSQKLEE KVTELSSETQ181RGETIKMTIT LKRELPSSSP VCIQVFNIIF RKILKKLSMY QIGRNFYNPS EPMEIPQHKL241SLWPGFAISV SYFERKLLFS ADVSYKVLRN ETVLEFMTAL CQRTGLSCFT QTCEKQLIGL301IVLTRYNNRT YSIDDIDWSV KPTHTFQKRD GTEITYVDYY KQQYDITVSD LNQPMLVSLL361KKKRNDNSEA QLAHLIPELC FLTGLTDQAT SDFQLMKAVA EKTRLSPSGR QQRLARLVDN421IQRNTNARFE LETWGLHFGS QISLTGRIVP SEKILMQDHI CQPVSAADWS KDIRTCKILN481AQSLNTWLIL CSDRTEYVAE SFLNCLRRVA GSMGFNVDYP KIIKVQENPA AFVRAIQQYV541DPDVQLVMCI LPSNQKTYYD SIKKYLSSDC PVPSQCVLAR TLNKQGMMMS IATKIAMQMT601CKLGGELWAV EIPLKSLMVV GIDVCKDALS KDVMVVGCVA SVNPRITRWF SRCILQRTMT661DVADCLKVFM TGALNKWYKY NHDLPARIIV YRAGVGDGQL KTLIEYEVPQ LLSSVAESSS721NTSSRLSVIV VRKKCMPRFF TEMNRTVQNP PLGTVVDSEA TRNEWYDFYL ISQVACRGTV781SPTYYNVIYD DNGLKPDHMQ RLTFKLCHLY YNWPGIVSVP APCQYAHKLT FLVAQSIHKE841PSLELANHLF YLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
852 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.