HISPPD2A / VIP1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113783-100
Catalog Number: LS-G113783
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HISPPD2A(PPIP5K1) (NM_014659), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HISPPD2A | IP6 kinase | IP6K | InsP6 and PP-IP5 kinase 1 | PPIP5K1 | VIP1 | HsVIP1 | IPS1 | KIAA0377 | VIP1 homolog
- Origin Species
- Human
- NCBI GenBank
- NM_014659
- SwissProt
- Q6PFW1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 156.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MWSLTASEGE STTAHFFLGA GDEGLGTRGI GMRPEESDSE LLEDEEDEVP PEPQIIVGIC61AMTKKSKSKP MTQILERLCR FDYLTVVILG EDVILNEPVE NWPSCHCLIS FHSKGFPLDK121AVAYSKLRNP FLINDLAMQY YIQDRREVYR ILQEEGIDLP RYAVLNRDPA RPEECNLIEG181EDQVEVNGAV FPKPFVEKPV SAEDHNVYIY YPSSAGGGSQ RLFRKIGSRS SVYSPESSVR241KTGSYIYEEF MPTDGTDVKV YTVGPDYAHA EARKSPALDG KVERDSEGKE IRYPVMLTAM301EKLVARKVCV AFKQTVCGFD LLRANGHSFV CDVNGFSFVK NSMKYYDDCA KILGNTIMRE361LAPQFQIPWS IPTEAEDIPI VPTTSGTMME LRCVIAIIRH GDRTPKQKMK MEVKHPRFFA421LFEKHGGYKT GKLKLKRPEQ LQEVLDITRL LLAELEKEPG GEIEEKTGKL EQLKSVLEMY481GHFSGINRKV QLTYYPHGVK ASNEGQDPQR ETLAPSLLLV LKWGGELTPA GRVQAEELGR541AFRCMYPGGQ GDYAGFPGCG LLRLHSTFRH DLKIYASDEG RVQMTAAAFA KGLLALEGEL601TPILVQMVKS ANMNGLLDSD GDSLSSCQHR VKARLHHILQ QDAPFGPEDY DQLAPTRSTS661LLNSMTIIQN PVKVCDQVFA LIENLTHQIR ERMQDPRSVD LQLYHSETLE LMLQRWSKLE721RDFRQKSGRY DISKIPDIYD CVKYDVQHNG SLGLQGTAEL LRLSKALADV VIPQEYGISR781EEKLEIAVGF CLPLLRKILL DLQRTHEDES VNKLHPLYSR GVLSPGRHVR TRLYFTSESH841VHSLLSVFRY GGLLDETQDA QWQRALDYLS AISELNYMTQ IVIMLYEDNT QDPLSEERFH901VELHFSPGVK GVEEEGSAPA GCGFRPASSE NEEMKTNQGS MENLCPGKAS DEPDRALQTS961PQPPEGPGLP RRSPLIRNRK AGSMEVLSET SSSRPGGYRL FSSSRPPTEM KQSGLGSQCT1021GLFSTTVLGG SSSAPNLQDY ARSHGKKLPP ASLKHRDGFE GCSMVPTIYP LETLHNALSL1081RQVSEFLSRV CQRHTDAQAQ ASAALFDSMH SSQASDNPFS PPRTLHSPPL QLQQRSEKPP1141WYSSGPSSTV SSAGPSSPTT VDGNSQFGFS DQPSLNSHVA EEHQGLGLLQ ETPGSGAQEL1201SIEGEQELFE PNQSPQVPPM ETSQPYEEVS QPCQEVPDIS QPCQDISEAL SQPCQKVPDI1261SQQCQENHDN GNHTCQEVPH ISQPCQKSSQ LCQKVSEEVC QLCLENSEEV SQPCQGVSVE1321VGKLVHKFHV GVGSLVQETL VEVGSPAEEI PEEVIQPYQE FSVEVGRLAQ ETSAINLLSQ1381GIPEIDKPSQ EFPEEIDLQA QEVPEEINSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
1408 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.