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HIRIP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HIRIP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G117205-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HIRIP3 (NM_003609), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HIRA-interacting protein 3 | HIRA interacting protein 3 | HIRIP3
Origin Species
Human
NCBI GenBank
NM_003609
SwissProt
Q9BW71

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAREKEMQEF TRSFFRGRPD LSTLTHSIVR RRYLAHSGRS HLEPEEKQAL KRLVEEELLK
61MQVDEAASRE DKLDLTKKGK RPPTPCSDPE RKRFRFNSES ESGSEASSPD YFGPPAKNGV
121AAEVSPAKEE NPRRASKAVE ESSDEERQRD LPAQRGEESS EEEEKGYKGK TRKKPVVKKQ
181APGKASVSRK QAREESEESE AEPVQRTAKK VEGNKGTKSL KESEQESEEE ILAQKKEQRE
241EEVEEEEKEE DEEKGDWKPR TRSNGRRKSA REERSCKQKS QAKRLLGDSD SEEEQKEAAS
301SGDDSGRDRE PPVQRKSEDR TQLKGGKRLS GSSEDEEDSG KGEPTAKGSR KMARLGSTSG
361EESDLEREVS DSEAGGGPQG ERKNRSSKKS SRKGRTRSSS SSSDGSPEAK GGKAGSGRRG
421EDHPAVMRLK RYIRACGAHR NYKKLLGSCC SHKERLSILR AELEALGMKG TPSLGKCRAL
481KEQREEAAEV ASLDVANIIS GSGRPRRRTA WNPLGEAAPP GELYRRTLDS DEERPRPAPP
541DWSHMRGIIS SDGESNTRTR PLEQKLISEE DLAANDILDY KDDDDKV

587 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.