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HIPK1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HIPK1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G104497-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HIPK1 (NM_181358), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HIPK1 | Nbak2 | Myak | KIAA0630
Origin Species
Human
NCBI GenBank
NM_152696
SwissProt
Q86Z02

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
87.1 kda
Protein Type
Lysate
Amino Acid Sequence
1MVLMFQIRYI SQTQGLPAEY LLSAGTKTTR FFNRDPNLGY PLWRLKTPEE HELETGIKSK
61EARKYIFNCL DDMAQVNMST DLEGTDMLAE KADRREYIDL LKKMLTIDAD KRITPLKTLN
121HQFVTMTHLL DFPHSNHVKS CFQNMEICKR RVHMYDTVSQ IKSPFTTHVA PNTSTNLTMS
181FSNQLNTVHN QASVLASSST AAAATLSLAN SDVSLLNYQS ALYPSSAAPV PGVAQQGVSL
241QPGTTQICTQ TDPFQQTFIV CPPAFQTGLQ ATTKHSGFPV RMDNAVPIVP QAPAAQPLQI
301QSGVLTQGSC TPLMVATLHP QVATITPQYA VPFTLSCAAG RPALVEQTAA VLQAWPGGTQ
361QILLPSTWQQ LPGVALHNSV QPTAMIPEAM GSGQQLADWR NAHSHGNQYS TIMQQPSLLT
421NHVTLATAQP LNVGVAHVVR QQQSSSLPSK KNKQSAPVSS KSSLDVLPSQ VYSLVGSSPL
481RTTSSYNSLV PVQDQHQPII IPDTPSPPVS VITIRSDTDE EEDNKYKPSS SGLKPRSNVI
541SYVTVNDSPD SDSSLSSPYS TDTLSALRGN SGSVLEGPGR VVADGTGTRT IIVPPLKTQL
601GDCTVATQAS GLLSNKTKPV ASVSGQSSGC CITPTGYRAQ RGGTSAAQPL NLSQNQQSSV
661APTSQERSSN PAPRRQQAFV APLSQAPYTF QHGSPLHSTG HPHLAPAPAH LPSQAHLYTY
721AAPTSAAALG STSSIAHLFS PQGSSRHAAA YTTHPSTLVH QVPVSVGPSL LTSASVAPAQ
781YQHQFATQSY IGSSRGSTIY TGYPLSPTKI SQYSYL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

816 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.