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HIPK1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HIPK1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G103762-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HIPK1 (NM_198269), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HIPK1 | Nbak2 | Myak | KIAA0630
Origin Species
Human
NCBI GenBank
NM_152696
SwissProt
Q86Z02

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
89.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MWSLGCVIAE LFLGWPLYPG ASEYDQIRYI SQTQGLPAEY LLSAGTKTTR FFNRDPNLGY
61PLWRLKTPEE HELETGIKSK EARKYIFNCL DDMAQVNMST DLEGTDMLAE KADRREYIDL
121LKKMLTIDAD KRITPLKTLN HQFVTMTHLL DFPHSNHVKS CFQNMEICKR RVHMYDTVSQ
181IKSPFTTHVA PNTSTNLTMS FSNQLNTVHN QASVLASSST AAAATLSLAN SDVSLLNYQS
241ALYPSSAAPV PGVAQQGVSL QPGTTQICTQ TDPFQQTFIV CPPAFQTGLQ ATTKHSGFPV
301RMDNAVPIVP QAPAAQPLQI QSGVLTQGSC TPLMVATLHP QVATITPQYA VPFTLSCAAG
361RPALVEQTAA VLQAWPGGTQ QILLPSTWQQ LPGVALHNSV QPTAMIPEAM GSGQQLADWR
421NAHSHGNQYS TIMQQPSLLT NHVTLATAQP LNVGVAHVVR QQQSSSLPSK KNKQSAPVSS
481KSSLDVLPSQ VYSLVGSSPL RTTSSYNSLV PVQDQHQPII IPDTPSPPVS VITIRSDTDE
541EEDNKYKPSS SGLKPRSNVI SYVTVNDSPD SDSSLSSPYS TDTLSALRGN SGSVLEGPGR
601VVADGTGTRT IIVPPLKTQL GDCTVATQAS GLLSNKTKPV ASVSGQSSGC CITPTGYRAQ
661RGGTSAAQPL NLSQNQQSSA APTSQERSSN PAPRRQQAFV APLSQAPYTF QHGSPLHSTG
721HPHLAPAPAH LPSQAHLYTY AAPTSAAALG STSSIAHLFS PQGSSRHAAA YTTHPSTLVH
781QVPVSVGPSL LTSASVAPAQ YQHQFATQSY IGSSRGSTIY TGYPLSPTKI SQYSYL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

836 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.