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HINFP Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HINFP Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G113161-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HINFP (NM_015517), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DKFZP434F162 | MBD2-interacting zinc finger 1 | MBD2-interacting zinc finger | MIZF | HiNF-P | Histone nuclear factor P | ZNF743 | HINFP
Origin Species
Human
NCBI GenBank
NM_015517
SwissProt
Q9BQA5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPPPGKVPRK ENLWLQCEWG SCSFVCSTME KFFEHVTQHL QQHLHGSGEE EEEEEEDDPL
61EEEFSCLWQE CGFCSLDSSA DLIRHVYFHC YHTKLKQWGL QALQSQADLG PCILDFQSRN
121VIPDIPDHFL CLWEHCENSF DNPEWFYRHV EAHSLCCEYE AVGKDNPVVL CGWKGCTCTF
181KDRSKLREHL RSHTQEKVVA CPTCGGMFAN NTKFLDHIRR QTSLDQQHFQ CSHCSKRFAT
241ERLLRDHMRN HVNHYKCPLC DMTCPLPSSL RNHMRFRHSE DRPFKCDCCD YSCKNLIDLQ
301KHLDTHSEEP AYRCDFENCT FSARSLCSIK SHYRKVHEGD SEPRYKCHVC DKCFTRGNNL
361TVHLRKKHQF KWPSGHPRFR YKEHEDGYMR LQLVRYESVE LTQQLLRQPQ EGSGLGTSLN
421ESSLQGIILE TVPGEPGRKE EEEEGKGSEG TALSASQDNP SSVIHVVNQT NAQGQQEIVY
481YVLSEAPGEP PPVPEPPSGG IMEKLQGIAE EPEIQMVTRT RPLEQKLISE EDLAANDILD
541YKDDDDKV

548 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.