Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HID1 / C17orf28 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101950-100
Catalog Number: LS-G101950
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HID1 (NM_030630), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C17orf28 | HID1 | HID1 domain containing | HID-1
- Origin Species
- Human
- SwissProt
- Q8IV36
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 88.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGSTDSKLNF RKAVIQLTTK TQPVEATDDA FWDQFWADTA TSVQDVFALV PAAEIRAVRE61ESPSNLATLC YKAVEKLVQG AESGCHSEKE KQIVLNCSRL LTRVLPYIFE DPDWRGFFWS121TVPGAGRGGQ GEEDDEHARP LAESLLLAIA DLLFCPDFTV QSHRRSTVDS AEDVHSLDSC181EYIWEAGVGF AHSPQPNYIH DMNRMELLKL LLTCFSEAMY LPPAPESGST NPWVQFFCST241ENRHALPLFT SLLNTVCAYD PVGYGIPYNH LLFSDYREPL VEEAAQVLIV TLDHDSASSA301SPTVDGTTTG TAMDDADPPG PENLFVNYLS RIHREEDFQF ILKGIARLLS NPLLQTYLPN361STKKIQFHQE LLVLFWKLCD FNKKFLFFVL KSSDVLDILV PILFFLNDAR ADQSRVGLMH421IGVFILLLLS GERNFGVRLN KPYSIRVPMD IPVFTGTHAD LLIVVFHKII TSGHQRLQPL481FDCLLTIVVN VSPYLKSLSM VTANKLLHLL EAFSTTWFLF SAAQNHHLVF FLLEVFNNII541QYQFDGNSNL VYAIIRKRSI FHQLANLPTD PPTIHKALQR RRRTPEPLSR TGSQEGTSME601GSRPAAPAEP GTLKTSLVAT PGIDKLTEKS QVSEDGTLRS LEPEPQQSLE DGSPAKGEPS661QAWREQRRPS TSSASGQWSP TPEWVLSWKS KLPLQTIMRL LQVLVPQVEK ICIDKGLTDE721SEILRFLQHG TLVGLLPVPH PILIRKYQAN SGTAMWFRTY MWGVIYLRNV DPPVWYDTDV781KLFEIQRVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
788 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.