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HIATL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HIATL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all HIATL1 antibodies →
$504.00each
Catalog Number: LS-G108740-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HIATL1(MFSD14B) (NM_032558), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HIATL1
Origin Species
Human
NCBI GenBank
NM_032558
SwissProt
Q5SR56

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPEKRAGAQA AGSTWLQGFG RPSVYHAAIV IFLEFFAWGL LTTPMLTVLH ETFSQHTFLM
61NGLIQGVKGL LSFLSAPLIG ALSDVWGRKP FLLGTVFFTC FPIPLMRISP WWYFAMISVS
121GVFSVTFSVI FAYVADVTQE HERSTAYGWV SATFAASLVS SPAIGAYLSA SYGDSLVVLV
181ATVVALLDIC FILVAVPESL PEKMRPVSWG AQISWKQADP FASLKKVGKD STVLLICITV
241FLSYLPEAGQ YSSFFLYLRQ VIGFGSVKIA AFIAMVGILS IVAQTAFLSI LMRSLGNKNT
301VLLGLGFQML QLAWYGFGSQ AWMMWAAGTV AAMSSITFPA ISALVSRNAE SDQQGVAQGI
361ITGIRGLCNG LGPALYGFIF YMFHVELTEL GPKLNSNNVP LQGAVIPGPP FLFGACIVLM
421SFLVALFIPE YSKASGVQKH SNSSSGSLTN TPERGSDEDI EPLLQDSSIW ELSSFEEPGN
481QCTEL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

485 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.