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HHLA1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HHLA1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G126517-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HHLA1 (NM_001145095), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HHLA1 | PLA2L | HERV-H LTR-associating 1
Origin Species
Human
NCBI GenBank
NM_001145095
SwissProt
C9JL84

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLGFLSRGPS MKLCMGLACV LSLWNTVSGI KGEAKKEKGM TFLPTTVSGL REEERKEKGV
61AFLATTELPA RSIDLSALNL TELVNGMLSR ALKDSKKFFS LLSVTSYSSF AFHKFSVAVY
121NISNLKTVDP AKFPTRYCYC LNNRTNDLSD FTALLVDIIG NSTSYLTEIF KSTSILSVNQ
181SNESDCIFIC VMTGKSGRNL SDFWEIEEKY PIINYTFTSG LSGVLGAATR GTARTSKPTT
241KSQKTLPSTS PGHWTQSTPW ASALRSSPWT ETAAPSETEE TLNTGRPPEL PARATATWFS
301ASHTLPALAT RRVARTQWLT ADRQTWASIS SVPWAQTISE KKPGGSLWET RSSPPTTAGT
361EEAMNTTSLL APAAEIMATP GSPSQASPTL GAFTHGTQTP SPTKATAPRY PQTGDLSAEW
421PFTAGEEPVL VPRPHQVSRC PQPLFKVGAM AAAPLTLAIQ RLNPCLMELC QFFQQCLCMS
481QRSPRTEDMR YCLEYYSWFL KNATYICQRV KRVSHSHTLK QKCLENICKS V
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

531 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.