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HHIPL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HHIPL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G109788-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HHIPL2 (NM_024746), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HHIP-like 2 | RP11-378J18.1 | HHIP-like protein 2 | HHIP3 | HHIPL2 | KIAA1822-like | KIAA1822L
Origin Species
Human
NCBI GenBank
NM_024746
SwissProt
Q6UWX4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
80.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLRTSTPNLC GGLHCRAPWL SSGILCLCLI FLLGQVGLLQ GHPQCLDYGP PFQPPLHLEF
61CSDYESFGCC DQHKDRRIAA RYWDIMEYFD LKRHELCGDY IKDILCQECS PYAAHLYDAE
121NTQTPLRNLP GLCSDYCSAF HSNCHSAISL LTNDRGLQES HGRDGTRFCH LLDLPDKDYC
181FPNVLRNDYL NRHLGMVAQD PQGCLQLCLS EVANGLRNPV SMVHAGDGTH RFFVAEQVGV
241VWVYLPDGSR LEQSFLDLKN IVLTTPWIGD ERGFLGLAFH PKFRHNRKFY IYYSCLDKKK
301VEKIRISEMK VSRADPNKAD LKSERVILEI EEPASNHNGG QLLFGLDGYM YIFTGDGGQA
361GDPFGLFGNA QNKSSLLGKV LRIDVNRAGS HGKRYRVPSD NPFVSEPGAH PAIYAYGIRN
421MWRCAVDRGD PITRQGRGRI FCGDVGQNRF EEVDLILKGG NYGWRAKEGF ACYDKKLCHN
481ASLDDVLPIY AYGHAVGKSV TGGYVYRGCE SPNLNGLYIF GDFMSGRLMA LQEDRKNKKW
541KKQDLCLGST TSCAFPGLIS THSKFIISFA EDEAGELYFL ATSYPSAYAP RGSIYKFVDP
601SRRAPPGKCK YKPVPVRTKS KRIPFRPLAK TVLDLLKEQS EKAARKSSSA TLASGPAQGL
661SEKGSSKKLA SPTSSKNTLR GPGTKKKARV GPHVRQGKRR KSLKSHSGRM RPSAEQKRAG
721RSLP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

724 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.