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HHIPL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HHIPL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G124593-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HHIPL1 (NM_001127258), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ARAR9245 | HHIPL1 | HHIP-like protein 1 | HHIP2 | HHIP-like 1 | UNQ9245 | KIAA1822
Origin Species
Human
NCBI GenBank
NM_001127258
SwissProt
Q96JK4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
86.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MARARAGALL ALWVLGAAAH PQCLDFRPPF RPTQPLRLCA QYSDFGCCDE GRDAELTRRF
61WALASRVDAA EWAACAGYAR DLLCQECSPY AAHLYDAEDP FTPLRTVPGL CQDYCLDMWH
121KCRGLFRHLS TDQELWALEG NLARFCRYLS LDDTDYCFPY LLVNKNLNSN LGHVVADAKG
181CLQLCLEEVA NGLRNPVAMV HARDGTHRFF VAEQVGLVWA YLPDRSRLGK PFLNISRVVL
241TSPWEGDERG FLGIAFHPSF QHNRRLYVYY SVGIRSSEWI RISEFRVSED DENAVDHSSE
301RIILEVKEPA SNHNGGQLLF GDDGYLYIFT GDGGMAGDPF GTFGNAQNKS ALLGKVLRID
361VDRKERGLPY GIPPDNPFVG DPAAQPEVYA LGVRNMWRCS FDRGDPSSGT GRGRLFCGDV
421GQNKFEEVDV VERGGNYGWR AREGFECYDR SLCANTSLND LLPIFAYPHT VGKSVTGGYV
481YRGCEYPNLN GLYIFGDFMS GRLMSLQENP GTGQWQYSEI CMGHGQTCEF PGLINNYYPY
541IISFGEDEAG ELYFMSTGEP SATAPRGVVY KIIDASRRAP PGKCQIQPAQ VKIRSRLIPF
601VPKEKFIPKT RSTPRPTARA PTRAPRRGRP TAAPPAPTPR PARPTQQPGS RRGGGRRRGR
661LNSASRAFRD GEVRLVRPAG LSSGSGRVEV FVGGRWGTVC DDSWNISGAA VVCRQLGFAY
721AVRAVKRAEF GQGGSLPILL DDVRCAGWER NLLECQHNGV GTHNCEHDED AGVVCSHQNP
781DL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

782 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.