Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HGH1 / FAM203A Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G112631-100
Catalog Number: LS-G112631
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C8orf30A(HGH1) (NM_016458), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BRP16 | FAM203A | LOC51236 | Protein FAM203A | Brain protein 16 | C8orf30A
- Origin Species
- Human
- NCBI GenBank
- NM_016458
- SwissProt
- Q9BTY7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 41.9 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MGEAGAGAGA SGGPEASPEA EVVKLLPFLA PGARADLQAA AVRHVLALTG CGPGRALLAG61QAALLQALME LAPASAPARD AARALVNLAA DPGLHETLLA ADPGLPARLM GRALDPQWPW121AEEAAAALAN LSREPAPCAA LMAALAAAEP ADSGLERLVR ALCTPGYNAR APLHYLAPLL181SNLSQRPAAR AFLLDPDRCV VQRLLPLTQY PDSSVRRGGV VGTLRNCCFE HRHHEWLLGP241EVDILPFLLL PLAGPEDFSE EEMERLPVDL QYLPPDKQRE PDADIRKMLV EAIMLLTATA301PGRQQVRDQG AYLILRELHS WEPEPDVRTA CEKLIQVLIG DEPERGMENL LEVQVPEDVE361QQLQQLDCRE QEQLERELAP EPWVERATPTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
390 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.