Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HFE2 / Hemojuvelin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106716-100
Catalog Number: LS-G106716
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Repulsive Guidance Molecule C(HFE2) (NM_145277), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Haemojuvelin | HFE2A | HJV | Hemochromatosis type 2 protein | Repulsive guidance molecule c | RGM domain family member C | Hemojuvelin | HFE2 | JH | RGMC
- Origin Species
- Human
- SwissProt
- Q6ZVN8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 33.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MIQHNCSRQG PTAPPPPRGP ALPGAGSGLP APDPCDYEGR FSRLHGRPPG FLHCASFGDP61HVRSFHHHFH TCRVQGAWPL LDNDFLFVQA TSSPMALGAN ATATRKLTII FKNMQECIDQ121KVYQAEVDNL PVAFEDGSIN GGDRPGGSSL SIQTANPGNH VEIQAAYIGT TIIIRQTAGQ181LSFSIKVAED VAMAFSAEQD LQLCVGGCPP SQRLSRSERN RRGAITIDTA RRLCKEGLPV241EDAYFHSCVF DVLISGDPNF TVAAQAALED ARAFLPDLEK LHLFPSDAGV PLSSATLLAP301LLSGLFVLWL CIQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
313 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.