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HEXDC Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HEXDC Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G105244-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HEXDC (NM_173620), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Beta-hexosaminidase D | Beta-N-acetylhexosaminidase | HEXDC | Hexosaminidase D
Origin Species
Human
NCBI GenBank
D60335
SwissProt
Q8WVB3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEFVLKHTAF AHLREVGSFP CTLNPHEAES LALVGAMIDQ VLELHPGAQR LHIGCDEVYY
61LGEGEASRRW LQQEQNSTGK LCLSHMRAVA SGVKARRPSV TPLVWDDMLR DLPEDQLAAS
121GVPQLVEPVL WDYTADLDVY GKVLLMQKYR RCGFPQLWAA SAFKGATGPS QAVPPVEHHL
181RNHVQWLQVA GSGPTDSLQG IILTGWQRYD HYSVLCELLP AGVPSLAACL QLLLRGGFDE
241DVKAKVENLL GISSLEKTDP VRQAPCSPPC PLLPLPFPRP WRQLFSAGLS AGRGPAPSLA
301ATSLPLSHKS ASICAALWMR CWRATGMSLA GSAPTTASGS SSTRSWFSTS SPQRSASWHS
361GAPSCRSWRL PCSWLSTRMP WRSGWRKTCT PACSGCKLCC RTSARCLPPR CHPPALAGTL
421LRTPEGRAHA RGLLLEAGGA LHCQMAWAIR AHVGVVPSGP AVSCPHSVPE GPGQPLGERL
481ENTEGSSTGR PAL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

493 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.