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HEPHL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HEPHL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G119291-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HEPHL1 (NM_001098672), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HEPHL1 | Hephaestin-like 1 | Hephaestin-like protein 1
Origin Species
Human
NCBI GenBank
NM_001098672
SwissProt
Q6MZM0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
131.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPRKQPAGCI FLLTFLGLSG LVGTVTRTYY IGIVEEYWNY VPQGKNVITG KSFTEDKLAT
61LFLERGPNRI GSIYKKAVYR RFTDGTYSIE IPKPPWLGFL GPILRAEVGD VIVIHLKNFA
121SRPYSLHPHG VFYNKDSEGA LYPDGTSGRN KNDDMVPPGK NYTYVWPVRE EYAPTPADAN
181CLTWVYHSHI DAPKDICSGL IGPLLVCKEG ILNRYSGTRN DVDREFVIMF TLVDENQSWY
241LNENIKHFCT NPDSVDKKDA VFQRSNKMHA LNGYLFGNFP EPDMCVGESV SWHLFGMGNE
301IDIHSIYFYG NTFISRGHRT DVVNLFPATF LTTEMIAENP GKWMITCQVS DHLQAGMLGQ
361YNVDNCKSDI FYPKMKGQQR RYFIAAEKIL WDYAPQGYNK FSGLPLNASG SDSDLYFTQG
421DNRIGGKYWK VRYTEFVDAT FTKRKRLSAE EAHLGILGPV IKAEVGDTLL VTFANKADKV
481YSILPHGVIY DKASDAAPNL DGFVKPGAHV KPGETFTYKW TVPESVSPTA GDPPCLTYLY
541FSAVDPIKDT SSGLVGPLLV CKKGVLNADG TQKGIDKEFY LLFTVFDENL SRYFDENIQK
601FIWHPFSIDK EDKEFVKSNR MHAVNGYMYG NQPGLNMCKR DRVSWHLIGL GTDTDMHGIV
661FQGNTIHLRG THRDSLALFP HMATTAFMQP DHAGIFRVFC ATMPHLSRGM GQIYEVSSCD
721NRDPSEQRYG MIRTFYIAAE EVEWDYAPNK NWEFEKQHVD ARGERHGDIF MNRTENWIGS
781QYKKVVYREY TDGEFVEIKA RPPREEHLEL LGPMIHAEVG NTVLIIFKNK ASRPYSISAQ
841GVEEMDSGKQ FQVPMTKPGE VKTYRWNIPK RSGPGPSDPN CIPWVYYSTV NFVKDTYSGL
901MGPLITCRKG VLNEKGRRSD VDYEFALLFL VFNENESWYL DDNIKKYLNK DPRDFKRTDD
961FEESNRMHAI NGKIFGNLHG LIMNEDTMTN WYLLGIGSEV DIHTIHYHAE SFLFKIDKSY
1021REDVYDLFPG TFQTIELFAD HPGTWLLHCH VSDHIHAGME TTYTVLRNID NRIPYSTTSP
1081GVASHPATVP SNERPGKEQL YFFGKNLGPT GAKAALVILF IIGLLLLITT VILSLRLCSA
1141MKQTDYQQVQ SCALPTDAL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1159 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.