Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HEPH / Hephaestin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113675-100
Catalog Number: LS-G113675
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Hephaestin(HEPH) (NM_014799), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Hephaestin | CPL | HEPH | KIAA0698
- Origin Species
- Human
- NCBI GenBank
- NM_014799
- SwissProt
- Q9BQS7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 130.45 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MESGHLLWAL LFMQSLWPQL TDGATRVYYL GIRDVQWNYA PKGRNVITNQ PLDSDIVASS61FLKSDKNRIG GTYKKTIYKE YKDDSYTDEV AQPAWLGFLG PVLQAEVGDV ILIHLKNFAT121RPYTIHPHGV FYEKDSEGSL YPDGSSGPLK ADDSVPPGGS HIYNWTIPEG HAPTDADPAC181LTWIYHSHVD APRDIATGLI GPLITCKRGA LDGNSPPQRQ DVDHDFFLLF SVVDENLSWH241LNENIATYCS DPASVDKEDE TFQESNRMHA INGFVFGNLP ELNMCAQKRV AWHLFGMGNE301IDVHTAFFHG QMLTTRGHHT DVANIFPATF VTAEMVPWEP GTWLISCQVN SHFRDGMQAL361YKVKSCSMAP PVDLLTGKVR QYFIEAHEIQ WDYGPMGHDG STGKNLREPG SISDKFFQKS421SSRIGGTYWK VRYEAFQDET FQEKMHLEED RHLGILGPVI RAEVGDTIQV VFYNRASQPF481SMQPHGVFYE KDYEGTVYND GSSYPGLVAK PFEKVTYRWT VPPHAGPTAQ DPACLTWMYF541SAADPIRDTN SGLVGPLLVC RAGALGADGK QKGVDKEFFL LFTVLDENKS WYSNANQAAA601MLDFRLLSED IEGFQDSNRM HAINGFLFSN LPRLDMCKGD TVAWHLLGLG TETDVHGVMF661QGNTVQLQGM RKGAAMLFPH TFVMAIMQPD NLGTFEIYCQ AGSHREAGMR AIYNVSQCPG721HQATPRQRYQ AARIYYIMAE EVEWDYCPDR SWEREWHNQS EKDSYGYIFL SNKDGLLGSR781YKKAVFREYT DGTFRIPRPR TGPEEHLGIL GPLIKGEVGD ILTVVFKNNA SRPYSVHAHG841VLESTTVWPL AAEPGEVVTY QWNIPERSGP GPNDSACVSW IYYSAVDPIK DMYSGLVGPL901AICQKGILEP HGGRSDMDRE FALLFLIFDE NKSWYLEENV ATHGSQDPGS INLQDETFLE961SNKMHAINGK LYANLRGLTM YQGERVAWYM LAMGQDVDLH TIHFHAESFL YRNGENYRAD1021VVDLFPGTFE VVEMVASNPG TWLMHCHVTD HVHAGMETLF TVFSRTEHLS PLTVITKETE1081KAVPPRDIEE GNVKMLGMQI PIKNVEMLAS VLVAISVTLL LVVLALGGVV WYQHRQRKLR1141RNRRSILDDS FKLLSFKQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1158 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.