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HEMK1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HEMK1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G112816-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HEMK1 (NM_016173), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HEMK1 | HEMK | MTQ1 | HEMK homolog 7kb | M.HsaHemKP
Origin Species
Human
NCBI GenBank
NM_016173
SwissProt
Q9Y5R4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
38.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MELWGRMLWA LLSGPGRRGS TRGWAFSSWQ PQPPLAGLSS AIELVSHWTG VFEKRGIPEA
61RESSEYIVAH VLGAKTFQSL RPALWTQPLT SQQLQCIREL SSRRLQRMPV QYILGEWDFQ
121GLSLRMVPPV FIPRPETEEL VEWVLEEVAQ RSHAVGSPGS PLILEVGCGS GAISLSLLSQ
181LPQSRVIAVD KREAAISLTH ENAQRLRLQD RIWIIHLDMT SERSWTHLPW GPMDLIVSNP
241PYVFHQDMEQ LAPEIRSYED PAALDGGEEG MDIITHILAL APRLLKDSGS IFLEVDPRHP
301ELVSSWLQSR PDLYLNLVAV RRDFCGRPRF LHIRRSGPTR TRPLEQKLIS EEDLAANDIL
361DYKDDDDKV

369 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.