LSBio
HELZ Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HELZ Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all HELZ antibodies →
$725.00each
Catalog Number: LS-G113632-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HELZ (NM_014877), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DHRC | DRHC | HELZ | KIAA0054 | Helicase with zinc finger | HUMORF5
Origin Species
Human
NCBI GenBank
D29677
SwissProt
P42694

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
218.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEDRRAEKSC EQACESLKRQ DYEMALKHCT EALLSLGQYS MADFTGPCPL EIERIKIESL
61LYRIASFLQL KNYMQADEDC RHVLGEGLAK GEDAFRAVLC CMQLKGKLQP VSTILAKSLT
121GESLNGMVTK DLTRLKTLLS ETETATSNAL SGYHVEDLDE GSCNGWHFRP PPRGITSSEE
181YTLCKRFLEQ GICRYGAQCT SAHSQEELAE WQKRYASRLI KLKQQNENKQ LSGSYMETLI
241EKWMNSLSPE KVLSECIEGV KVEHNPDLSV TVSTKKSHQT WTFALTCKPA RMLYRVALLY
301DAHRPHFSII AISAGDSTTQ VSQEVPENCQ EWIGGKMAQN GLDHYVYKVG IAFNTEIFGT
361FRQTIVFDFG LEPVLMQRVM IDAASTEDLE YLMHAKQQLV TTAKRWDSSS KTIIDFEPNE
421TTDLEKSLLI RYQIPLSADQ LFTQSVLDKS LTKSNYQSRL HDLLYIEEIA QYKEISKFNL
481KVQLQILASF MLTGVSGGAK YAQNGQLFGR FKLTETLSED TLAGRLVMTK VNAVYLLPVP
541KQKLVQTQGT KEKVYEATIE EKTKEYIFLR LSRECCEELN LRPDCDTQVE LQFQLNRLPL
601CEMHYALDRI KDNGVLFPDI SMTPTIPWSP NRQWDEQLDP RLNAKQKEAV LAITTPLAIQ
661LPPVLIIGPY GTGKTFTLAQ AVKHILQQQE TRILICTHSN SAADLYIKDY LHPYVEAGNP
721QARPLRVYFR NRWVKTVHPV VHQYCLISSA HSTFQMPQKE DILKHRVVVV TLNTSQYLCQ
781LDLEPGFFTH ILLDEAAQAM ECETIMPLAL ATQNTRIVLA GDHMQLSPFV YSEFARERNL
841HVSLLDRLYE HYPAEFPCRI LLCENYRSHE AIINYTSELF YEGKLMASGK QPAHKDFYPL
901TFFTARGEDV QEKNSTAFYN NAEVFEVVER VEELRRKWPV AWGKLDDGSI GVVTPYADQV
961FRIRAELRKK RLSDVNVERV LNVQGKQFRV LFLSTVRTRH TCKHKQTPIK KKEQLLEDST
1021EDLDYGFLSN YKLLNTAITR AQSLVAVVGD PIALCSIGRC RKFWERFIAL CHENSSLHGI
1081TFEQIKAQLE ALELKKTYVL NPLAPEFIPR ALRLQHSGST NKQQQSPPKG KSLHHTQNDH
1141FQNDGIVQPN PSVLIGNPIR AYTPPPPLGP HPNLGKSPSP VQRIDPHTGT SILYVPAVYG
1201GNVVMSVPLP VPWTGYQGRF AVDPRIITHQ AAMAYNMNLL QTHGRGSPIP YGLGHHPPVT
1261IGQPQNQHQE KDQHEQNRNG KSDTNNSGPE INKIRTPEKK PTEPKQVDLE SNPQNRSPES
1321RPSVVYPSTK FPRKDNLNPR HINLPLPAPH AQYAIPNRHF HPLPQLPRPP FPIPQQHTLL
1381NQQQNNLPEQ PNQIPPQPNQ VVQQQSQLNQ QPQQPPPQLS PAYQAGPNNA FFNSAVAHRP
1441QSPPAEAVIP EQQPPPMLQE GHSPLRAIAQ PGPILPSHLN SFIDENPSGL PIGEALDRIH
1501GSVALETLRQ QQARFQQWSE HHAFLSQGSV PYPHHHHPHL QHLPQPPLGL HQPPVRADWK
1561LTSSAEDEVE TTYSRFQDLI RELSHRDQSE TRELAEMPPP QSRLLQYRQV QSRSPPAVPS
1621PPSSTDHSSH FSNFNDNSRD IEVASNPAFP QRLPPQIFNS PFSLPSEHLA PPPLKYLAPD
1681GAWTFANLQQ NHLMGPGFPY GLPPLPHRPP QNPFVQIQNH QHAIGQEPFH PLSSRTVSSS
1741SLPSLEEYEP RGPGRPLYQR RISSSSVQPC SEEVSTPQDS LAQCKELQDH SNQSSFNFSS
1801PESWVNTTSS TPYQNIPCNG SSRTAQPREL IAPPKTVKPP EDQLKSENLE VSSSFNYSVL
1861QHLGQFPPLM PNKQIAESAN SSSPQSSAGG KPAMSYASAL RAPPKPRPPP EQAKKSSDPL
1921SLFQELSLGS SSGSNGFYSY FK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1942 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.