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HECW1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HECW1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all HECW1 antibodies →
$504.00each
Catalog Number: LS-G113488-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HECW1 (NM_015052), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HNEDL1 | HECT type E3 ubiquitin ligase | HECW1 | NEDL1 | KIAA0322
Origin Species
Human
NCBI GenBank
AB002320
SwissProt
Q76N89

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
179.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLLHLCSVKN LYQNRFLGLA AMASPSRNSQ SRRRCKEPLR YSYNPDQFHN MDLRGGPHDG
61VTIPRSTSDT DLVTSDSRST LMVSSSYYSI GHSQDLVIHW DIKEEVDAGD WIGMYLIDEV
121LSENFLDYKN RGVNGSHRGQ IIWKIDASSY FVEPETKICF KYYHGVSGAL RATTPSVTVK
181NSAAPIFKSI GADETVQGQG SRRLISFSLS DFQAMGLKKG MFFNPDPYLK ISIQPGKHSI
241FPALPHHGQE RRSKIIGNTV NPIWQAEQFS FVSLPTDVLE IEVKDKFAKS RPIIKRFLGK
301LSMPVQRLLE RHAIGDRVVS YTLGRRLPTD HVSGQLQFRF EITSSIHPDD EEISLSTEPE
361SAQIQDSPMN NLMESGSGEP RSEAPESSES WKPEQLGEGS VPDRPGNQSI ELSRPAEEAA
421VITEAGDQGM VSVGPEGAGE LLAQVQKDIQ PAPSAEELAE QLDLGEEASA LLLEDGEAPA
481STKEEPLEEE ATTQSRAGRE EEEKEQEEEG DVSTLEQGEG RLQLRASVKR KSRPCSLPVS
541ELETVIASAC GDPETPRTHY IRIHTLLHSM PSAQGGSAAE EEDGAEEEST LKDSSEKDGL
601SEVDTVAADP SALEEDREEP EGATPGTAHP GHSGGHFPSL ANGAAQDGDT HPSTGSESDS
661SPRQGGDHSC EGCDASCCSP SCYSSSCYST SCYSSSCYSA SCYSPSCYNG NRFASHTRFS
721SVDSAKISES TVFSSQDDEE EENSAFESVP DSMQSPELDP ESTNGAGPWQ DELAAPSGHV
781ERSPEGLESP VAGPSNRREG ECPILHNSQP VSQLPSLRPE HHHYPTIDEP LPPNWEARID
841SHGRVFYVDH VNRTTTWQRP TAAATPDGMR RSGSIQQMEQ LNRRYQNIQR TIATERSEED
901SGSQSCEQAP AGGGGGGGSD SEAESSQSSL DLRREGSLSP VNSQKITLLL QSPAVKFITN
961PEFFTVLHAN YSAYRVFTSS TCLKHMILKV RRDARNFERY QHNRDLVNFI NMFADTRLEL
1021PRGWEIKTDQ QGKSFFVDHN SRATTFIDPR IPLQNGRLPN HLTHRQHLQR LRSYSAGEAS
1081EVSRNRGASL LARPGHSLVA AIRSQHQHES LPLAYNDKIV AFLRQPNIFE MLQERQPSLA
1141RNHTLREKIH YIRTEGNHGL EKLSCDADLV ILLSLFEEEI MSYVPLQAAF HPGYSFSPRC
1201SPCSSPQNSP GLQRASARAP SPYRRDFEAK LRNFYRKLEA KGFGQGPGKI KLIIRRDHLL
1261EGTFNQVMAY SRKELQRNKL YVTFVGEEGL DYSGPSREFF FLLSQELFNP YYGLFEYSAN
1321DTYTVQISPM SAFVENHLEW FRFSGRILGL ALIHQYLLDA FFTRPFYKAL LRLPCDLSDL
1381EYLDEEFHQS LQWMKDNNIT DILDLTFTVN EEVFGQVTER ELKSGGANTQ VTEKNKKEYI
1441ERMVKWRVER GVVQQTEALV RGFYEVVDSR LVSVFDAREL ELVIAGTAEI DLNDWRNNTE
1501YRGGYHDGHL VIRWFWAAVE RFNNEQRLRL LQFVTGTSSV PYEGFAALRG SNGLRRFCIE
1561KWGKITSLPR AHTCFNRLDL PPYPSYSMLY EKLLTAVEET STFGLE
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

1606 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.