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HECTD2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HECTD2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all HECTD2 antibodies →
$504.00each
Catalog Number: LS-G102540-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HECTD2 (NM_182765), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HECT domain containing 2 | HECTD2
Origin Species
Human
NCBI GenBank
NM_173497

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
87.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSEAVRVPSP ATPLVVAAAA PEERKGKESE REKLPPIVSA GAGATAGLDR GAKGQISTFS
61SFISAVSPKK EAAENRSSPA HLVFLNIKNV REPPPICLDV RQKQRTSMDA SSSEMKAPVL
121PEPILPIQPK TVKDFQEDVE KVKSSGDWKA VHDFYLTTFD SFPELNAAFK KDATASFNTI
181EDSGINAKFV NAVYDTLLNT PQDVQKTVLK GIINSLLREW KGPRTKDDLR AYFILLQNPQ
241FNNTSTYVIY AHLLRQIATL VEADHHFLVH WFKKLSQKRF KQLVERLLQF ISLRLFPAKP
301EEFPPITKCS WWIPSAAKVL ALLNTANNLV HPPLIPYTDF YNSTLDHIDL MEEYHTWQNF
361GNSHRFSFCQ YPFVISVAAK KIIIQRDSEQ QMINIARQSL VDKVSRRQRP DMNILFLNMK
421VRRTHLVSDS LDELTRKRAD LKKKLKVTFV GEAGLDMGGL TKEWFLLLIR QIFHPDYGMF
481TYHKDSHCHW FSSFKCDNYS EFRLVGILMG LAVYNSITFD IRFPPCCYKK LLSPPIIPSG
541QNIPVGICNV TVDDLCQIMP ELAHGLSELL SHEGNVEEDF YSTFQVFQEE FGIIKSYNLK
601PGGDKISVTN QNRKEYVQLY TDFLLNKSIY KQFAAFYYGF HSVCASNALM LLRPEEVEIL
661VCGSPDLDMH ALQRSTQYDG YAKTDLTIKY FWDVVLGFPL DLQKKLLHFT TGSDRVPVGG
721MADLNFKISK NETSTNCLPV AHTCFNQLCL PPYKSKKDLK QKLIIGISNS EGFGLE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

776 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.