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HECA Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HECA Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all HECA antibodies →
$504.00each
Catalog Number: LS-G112787-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HECA (NM_016217), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DJ225E12.1 | HDCL | Headcase homolog (Drosophila) | Headcase protein homolog | HECA | HHDC
Origin Species
Human
NCBI GenBank
NM_016217
SwissProt
Q9UBI9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPNPKNSKGG RKNKRANSSG DEQENGAGAL AAAGAAGAAA GGALAAAAGC GAAAAGAPGA
61GGAAGAGGAG TGAANAAAAA GAAAAGDAKN EAPCATPLIC SFGRPVDLEK DDYQKVVCNN
121EHCPCSTWMH LQCFYEWESS ILVQFNCIGR ARSWNEKQCR QNMWTKKGYD LAFRFCSCRC
181GQGHLKKDTD WYQVKRMQDE KKKKSGSEKN TGRPPGEAAE EAKKCRPPNK PQKGPSHDLP
241RRHSMDRQNS QEKAVGAAAY GARSPGGSPG QSPPTGYSIL SPAHFSGPRS SRYLGEFLKN
301AIHLEPHKKA MAGGHVFRNA HFDYSPAGLA VHRGGHFDTP VQFLRRLDLS ELLTHIPRHK
361LNTFHVRMED DAQVGQGEDL RKFILAALSA SHRNVVNCAL CHRALPVFEQ FPLVDGTLFL
421SPSRHDEIEY DVPCHLQGRL MHLYAVCVDC LEGVHKIICI KCKSRWDGSW HQLGTMYTYD
481ILAASPCCQA RLNCKHCGKP VIDVRIGMQY FSEYSNVQQC PHCGNLDYHF VKPFSSFKVL
541EAY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

543 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.