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HEATR9 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HEATR9 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all HEATR9 antibodies →
$725.00each
Catalog Number: LS-G106001-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C17orf66(HEATR9) (NM_152781), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C17orf66
Origin Species
Human
SwissProt
A2RTY3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAYEKSTDIS DVSRSMFLYP WLEYPDKTKE LRKAMAPVHL PLSCYQMPKE EFPPSPECWR
61QHPSKPNSVP YCYFKKPEIY THWHDLYDQR EEREAEKMLR KMRDDCRYIK EVHQTHIKMF
121HLPMSKLTIK SEMRSRPLEP TQDPLKWQRL RELTKSLESP REDEQFYAAQ ALGCLRISDK
181FVMEALQQVA QTGPEKVKYE AYRTLAILGC LNKHVIRALI KQLKEKNEGQ RMETLTGLRM
241ALNSWAAVSK DKRTQVGDEG KLVPVLQTLI KKSSSEASLE AALCLGFLRP CSNMVQEFLL
301QCLCQGLKTQ RMKALRMLVK VMHVHSAPVI KAILDQLCSS SVLEDRFEAT QMLKTIGLEQ
361IQAQGLEELT FNLLRRKTHN EPFLAVRQAV AQTVEELKLK PTMMNLVEAQ LMNPDATARQ
421EAVISLGVLG IRSPQVFHLL LDLLDAENHQ AVKKSLQETL ILCASIDPWI QNKLKNKVLS
481VYEAPKTNVK AEPTRFQKEP ENPEELTIQD FRLAKLNPLF IAKSITKVGQ KKTPAFPPCC
541SKPRKHRPQV IGPWQPRIKK QLRVLAEIAK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

570 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.