Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HEATR6 / ABC1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G110480-100
Catalog Number: LS-G110480
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HEATR6 (NM_022070), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ABC1 | Amplified in breast cancer 1 | HEATR6 | HEAT repeat containing 6
- Origin Species
- Human
- NCBI GenBank
- NM_022070
- SwissProt
- Q6AI08
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 128.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAVQVVGSW PSVQPREAPR EAIPERGNGF RLLSARLCAL RPDDSSSART EIHLLFDQLI61SENYSEGSGV APEDVSALLV QACRLVPLNQ NHLVSKVSQL IHHLLNRLQV IVDEQHLDFL121LAYTISAIHQ CSSWTHREIL QALAALVYCN GSKCQKYLPE LLGNTGLLMK LSDLAQSDPE181VRRAAVHCMA NLCLSVPGQP YLEEPYQNVC FQAFLTILQS PKSSDMDDIT FCMLLQNALK241GIQSLLNGGR MKLTQTDELG ALLAVLKKFM FHGLPGLNIE MPTVLYPTPL PQYDGRTPIK301PQQSESSASR PTLNKKKKSK VKPKKIQQGE EEEKESSGEI EAAPVTGTGR VNLHEGNTWC361PSSLGVQSLP LDGSGAAEKD GVSSSFSSSS WKRVSSSESD FSDAEGGMQS KMRSYQAKVR421QGALVCFLST IKSIEKKVLY GYWSAFIPDT PELGSPQSVS LMTLTLKDPS PKTRACALQV481LSAILEGSKQ FLSVAEDTSD HRRAFTPFSV MIACSIRELH RCLLLALVAE SSSQTVTQII541KCLANLVSNA PYDRLKLSLL TKVWNQIKPY IRHKDVNVRV SSLTLLGAIV STHAPLPEVQ601LLLQQPCSSG LGNSNSATPH LSPPDWWKKA PAGPSLEETS VSSPKGSSEP CWLIRLCISI661VVLPKEDSCS GSDAGSAAGS TYEPSPMRLE ALQVLTLLAR GYFSMTQAYL MELGEVICKC721MGEADPSIQL HGAKLLEELG TGLIQQYKPD STAAPDQRAP VFLVVMFWTM MLNGPLPRAL781QNSEHPTLQA SACDALSSIL PEAFSNLPND RQMLCITVLL GLNDSKNRLV KAATSRALGV841YVLFPCLRQD VIFVADAANA ILMSLEDKSL NVRAKAAWSL GNLTDTLIVN METPDPSFQE901EFSGLLLLKM LRSAIEASKD KDKVKSNAVR ALGNLLHFLQ PSHIEKPTFA EIIEESIQAL961ISTVLTEAAM KVRWNACYAM GNVFKNPALP LGTAPWTSQA YNALTSVVTS CKNFKVRIRS1021AAALSVPGKR EQYGSVDQYA RIWNALVTAL QKSEDTIDFL EFKYCVSLRT QICQALIHLL1081SLASASDLPC MKETLELSGN MVQSYILQFL KSGAEGDDTG APHSPQERDQ MVRMALKHMG1141SIQAPTGDTA RRAIMGFLEE ILAVCFDSSG SQGALPGLTN QTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1181 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.