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HEATR4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HEATR4 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all HEATR4 antibodies →
$504.00each
Catalog Number: LS-G103169-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HEATR4 (NM_203309), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HEAT repeat containing 4 | HEATR4
Origin Species
Human
SwissProt
Q86WZ0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
111.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVFFSSQYRL HRKSQYLKMA AANLTFSQEV VWQRGLPSIP YSQYSFDHLY NTNDIIHTPQ
61IRKARPQKPV SFKFLGSSSP LTGDTSLAVK TESSANPEKK LKKSKPASTV REAPRPLIHH
121PCMHPDMLGR PPSLDVNLEE REAWLLPPEK EARAWEATVL EKLNERTARW IQSKRPRRPG
181ASPNKWQSFL RQQYDWSHIR DELTSASDLE LLKQLEAEET AEFEDQSVIL PPQEKKKPEL
241LLPVYYRLPS YFQQAETVEI MPGNKSTEDI HEKTSLSQPQ TQSYFRQVTP RAGKFAYSTD
301NTFEQEIYFD EVQIIHQIGA KRDQIVLENL NRYNKQLSKV FPETPEKWSA QAIPEASYRP
361VQGALRWTAL PTPAKDMLLQ VGEKDVPIKT RRLKKQAKSL QEDVTWELVV LRRMLKEWKT
421AWALIIEWHH ETVENLLQSL GDLHDDVRIK AITTCATAAL ERPRIATSQR DSDKTIQDLP
481EVLLPALEAA LCDKNAHVRM AAAICQYAIQ SHNPLARNIM QTALLKGNSV DSWAAAQCLA
541LEGTATYPVI KRILHQLFTK KNEDTEEQSY ILLSYLSEKT TLIHTMLAVE LNSCQWKNRI
601VACQAFSRIS GNVCLDMKHK LIQLMWNDWN KEVRRAAAQA LGQMSLGKEV HDIIRVKLGQ
661GNSQERVEAL YLIGELKLMT AKLLPSFLHC FSDDFTAVRR AACLAAGALQ IRDKMVLECL
721LNLMQRDPYW KIKAFAIRAL GQIGQVSPEL TDLLLWAIHY EESPGVRLEA CRSILALKLQ
781GDRVRDTFLD VLLLENHDAV LKEMYQTMKI LNLGNEGNQE MLQEIKNRIK TLSQKDLLTH
841KILKLEMVMG KVREEAKRVY LKPKGEQGPL TLQTLLQETF QDEMVLPRRP SEVCDTEAVI
901KPVKPRAPNP WLQSSVPGLT TRSKVRSSLV KDLRTSPEKR IAVGPFRSDY PALYLGKFSE
961RTFFSPIMSS PSGKKGAHL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

979 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.