LSBio
HDX Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HDX Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all HDX antibodies →
$504.00each
Catalog Number: LS-G106910-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HDX (NM_144657), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CXorf43 | Highly divergent homeobox | GPHPX | HDX | PHP
Origin Species
Human
SwissProt
Q7Z353

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
77 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNLRSVFTVE QQRILQRYYE NGMTNQSKNC FQLILQCAQE TKLDFSVVRT WVGNKRRKMS
61SKNSESGTAT TGTSLSAPDI TVRNVVNIAR PSSQQSSWTS ANNDVIVTGI YSPASSSSRQ
121GTNKHTDTQI TEAHKIPIQK TATKNDTEFQ LHIPVQRQVA HCKNASLLLG EKTIILSRQT
181SVLNAGNSVF NHAKKNYGNS SVQASEMTVP QKPSVCHRPC KIEPVGIQRS YKPEHTGPAL
241HNLCGQKPTI RDPYCRTQNL EIREVFSLAV SDYPQRILGG NAPQKPSSAE GNCLSIAMET
301GDAEDEYARE EELASMRAQI PSYSRFYESG SSLRAENQST TLPGPGRNMP NSQMVNIRDM
361SDNVLYQNRN YHLTPRTSLH TASSTMYSNT NPLRSNFSPH FASSNQLRLS QNQNNYQISG
421NLTVPWITGC SRKRALQDRT QFSDRDLATL KKYWDNGMTS LGSVCREKIE AVATELNVDC
481EIVRTWIGNR RRKYRLMGIE VPPPRGGPAD FSEQPESGSL SALTPGEEAG PEVGEDNDRN
541DEVSICLSEG SSQEEPNEVV PNDARAHKEE DHHAVTTDNV KIEIIDDEES DMISNSEVEQ
601VNSFLDYKNE EVKFIENELE IQKQKYFKLQ TFVRSLILAM KADDKEQQQA LLSDLPPELE
661EMDFNHASLE PDDTSFSVSS LSEKNVSESL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

690 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.