Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HDS / DHDDS Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G109682-100
Catalog Number: LS-G109682
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Dehydrodolichyl Diphosphate Synthase(DHDDS) (NM_024887), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Cis-IPTase | Cis-isoprenyltransferase | Dedol-PP synthase | CPT | Cis-prenyl transferase | DHDDS | DS | HDS | RP59
- Origin Species
- Human
- NCBI GenBank
- NM_024887
- SwissProt
- Q86SQ9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 38.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MSWIKEGELS LWERFCANII KAGPMPKHIA FIMDGNRRYA KKCQVERQEG HSQGFNKLAE61TLRWCLNLGI LEVTVYAFSI ENFKRSKSEV DGLMDLARQK FSRLMEEKEK LQKHGVCIRV121LGDLHLLPLD LQELIAQAVQ ATKNYNKCFL NVCFAYTSRH EISNAVREMA WGVEQGLLDP181SDISESLLDK CLYTNRSPHP DILIRTSGEV RLSDFLLWQT SHSCLVFQPV LWPEYTFWNL241FEAILQFQMN HSMLQKARDM YAEERKRQQL ERDQATVTEQ LLREGLQASG DAQLRRTRLH301KLSARREERV QGFLQALELK RADWLARLGT ASATRTRPLE QKLISEEDLA ANDILDYKDD361DDKV
364 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.