LSBio
HDAC7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HDAC7 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G112509-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HDAC7A (NM_016596), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DKFZP586J0917 | HD7 | Histone deacetylase 7 | HDAC7 | Histone deacetylase 7A | HD7A | HDAC7A
Origin Species
Human
NCBI GenBank
NM_016596

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
99.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDLRVGQRPP VEPPPEPTLL ALQRPQRLHH HLFLAGLQQQ RSVEPMRLSM DTPMPELQVG
61PQEQELRQLL HKDKSKRSAV ASSVVKQKLA EVILKKQQAA LERTVHPNSP GIPYRTLEPL
121ETEGATRSML SSFLPPVPSL PSDPPEHFPL RKTVSEPNLK LRYKPKKSLE RRKNPLLRKE
181SAPPSLRRRP AETLGDSSPS SSSTPASGCS SPNDSEHGPN PILGDSDRRT HPTLGPRGPI
241LGSPHTPLFL PHGLEPEAGG TLPSRLQPIL LLDPSGSHAP LLTVPGLGPL PFHFAQSLMT
301TERLSGSGLH WPLSRTRSEP LPPSATAPPP PGPMQPRLEQ LKTHVQVIKR SAKPSEKPRL
361RQIPSAEDLE TDGGGPGQVV DDGLEHRELG HGQPEARGPA PLQQHPQVLL WEQQRLAGRL
421PRGSTGDTVL LPLAQGGHRP LSRAQSSPAA PASLSAPEPA SQARVLSSSE TPARTLPFTT
481GLIYDSVMLK HQCSCGDNSR HPEHAGRIQS IWSRLQERGL RSQCECLRGR KASLEELQSV
541HSERHVLLYG TNPLSRLKLD NGKLAGLLAQ RMFVMLPCGG VGVDTDTIWN ELHSSNAARW
601AAGSVTDLAF KVASRELKNG FAVVRPPGHH ADHSTAMGFC FFNSVAIACR QLQQQSKASK
661ILIVDWDVHH GNGTQQTFYQ DPSVLYISLH RHDDGNFFPG SGAVDEVGAG SGEGFNVNVA
721WAGGLDPPMG DPEYLAAFRI VVMPIAREFS PDLVLVSAGF DAAEGHPAPL GGYHVSAKCF
781GYMTQQLMNL AGGAVVLALE GGHDLTAICD ASEACVAALL GNRVDPLSEE GWKQKPNLNA
841IRSLEAVIRV HSKYWGCMQR LASCPDSWVP RVPGADKEEV EAVTALASLS VGILAEDRPS
901EQLVEEEEPM NL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

912 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.