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HDAC2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HDAC2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G118531-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HDAC2 (NM_001527), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HDAC2 | HD2 | Histone deacetylase 2 | RPD3 | YAF1 | YY1-associated factor 1
Origin Species
Human
NCBI GenBank
NM_001527

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
66 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRSPPCGLLR WFGGPLLASW CRCHLRFRAF GTSAGWYRAF PAPPPLLPPA CPSPRDYRPH
61VSLSPFLSRP SRGGSSSSSS SRRRSPVAAV AGEPMAYSQG GGKKKVCYYY DGDIGNYYYG
121QGHPMKPHRI RMTHNLLLNY GLYRKMEIYR PHKATAEEMT KYHSDEYIKF LRSIRPDNMS
181EYSKQMQRFN VGEDCPVFDG LFEFCQLSTG GSVAGAVKLN RQQTDMAVNW AGGLHHAKKS
241EASGFCYVND IVLAILELLK YHQRVLYIDI DIHHGDGVEE AFYTTDRVMT VSFHKYGEYF
301PGTGDLRDIG AGKGKYYAVN FPMRDGIDDE SYGQIFKPII SKVMEMYQPS AVVLQCGADS
361LSGDRLGCFN LTVKGHAKCV EVVKTFNLPL LMLGGGGYTI RNVARCWTYE TAVALDCEIP
421NELPYNDYFE YFGPDFKLHI SPSNMTNQNT PEYMEKIKQR LFENLRMLPH APGVQMQAIP
481EDAVHEDSGD EDGEDPDKRI SIRASDKRIA CDEEFSDSED EGEGGRRNVA DHKKGAKKAR
541IEEDKKETED KKTDVKEEDK SKDNSGEKTD TKGTKSEQLS NP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

582 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.