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HCFC2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HCFC2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all HCFC2 antibodies →
$504.00each
Catalog Number: LS-G114337-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Host cell factor C2(HCFC2) (NM_013320), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HCFC2 | Host cell factor 2 | Host cell factor C2 | HCF-2 | HCF2 | C2 factor
Origin Species
Human
NCBI GenBank
NM_013320
SwissProt
Q9Y5Z7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
86.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAPSLLNWR RVSSFTGPVP RARHGHRAVA IRELMIIFGG GNEGIADELH VYNTATNQWF
61LPAVRGDIPP GCAAHGFVCD GTRILVFGGM VEYGRYSNEL YELQASRWLW KKVKPHPPPS
121GLPPCPRLGH SFSLYGNKCY LFGGLANESE DSNNNVPRYL NDFYELELQH GSGVVGWSIP
181VTKGVVPSPR ESHTAVIYCK KDSGSPKMYV FGGMCGARLD DLWQLDLETM SWSKPETKGT
241VPLPRSLHTA SVIGNKMYIF GGWVPHKGEN TETSPHDCEW RCTSSFSYLN LDTTEWTTLV
301SDSQEDKKNS RPRPRAGHCA VAIGTRLYFW SGRDGYKKAL NSQVCCKDLW YLDTEKPPAP
361SQVQLIKATT NSFHVKWDEV STVEGYLLQL STDLPYQAAS SDSSAAPNMQ GVRMDPHRQG
421SNNIVPNSIN DTINSTKTEQ PATKETSMKN KPDFKALTDS NAILYPSLAS NASNHNSHVV
481DMLRKNEGPH TSANVGVLSS CLDVRTVIPE TSVSSTVSST QTMVTQQTIK TESSSTNGAV
541VKDETSLTTF STKSEVDETY ALPATKISRV ETHATATPFS KETPSNPVAT VKAGERQWCD
601VGIFKNNTAL VSQFYLLPKG KQSISKVGNA DVPDYSLLKK QDLVPGTGYR FRVAAINGCG
661IGPFSKISEF KTCIPGFPGA PSAVRISKNV EGIHLSWEPP TSPSGNILEY SAYLAIRTAQ
721IQDNPSQLVF MRIYCGLKTS CIVTAGQLAN AHIDYTSRPA IVFRISAKNE KGYGPATQVR
781WLQGNNKKAP LN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

792 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.