Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HC71 / GLOD4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G112878-100
Catalog Number: LS-G112878
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GLOD4 (NM_016080), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CGI-150 | C17orf25 | Glyoxalase domain containing 4 | HC71 | GLOD4
- Origin Species
- Human
- NCBI GenBank
- NM_016080
- SwissProt
- Q9HC38
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 33.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAARRALHFV FKVGNRFQTA RFYRDVLGMK VLRHEEFEEG CKAACNGPYD GKWSKTMVGF61GPEDDHFVAE LTYNYGVGDY KLGNDFMGIT LASSQAVSNA RKLEWPLTEV AEGVFETEAP121GGYKFYLQNR SLPQSDPVLK VTLAVSDLQK SLNYWCNLLG MKIYEKDEEK QRALLGYADN181QCKLELQGVK GGVDHAAAFG RIAFSCPQKE LPDLEDLMKR ENQKILTPLV SLDTPGKATV241QVVILADPDG HEICFVGDEA FRELSKMDPE GSKLLDDAMA ADKSDEWFAK HNKPKASGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
298 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.