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HAUS7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HAUS7 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G112404-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human UCHL5IP(HAUS7) (NM_017518), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HAUS7 | UCHL5-interacting protein | UCHL5IP | UIP1 | UCHL5 interacting protein | X-linked protein STS1769
Origin Species
Human
NCBI GenBank
NM_017518
SwissProt
Q99871

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
40.6 kda
Protein Type
Lysate
Amino Acid Sequence
1MGGARLGARN MAGQDAGCGR GGDDYSEDEG DSSVSRAAVE VFGKLKDLNC PFLEGLYITE
61PKTIQELLCS PSEYRLEILE WMCTRVWPSL QDRFSSLKGV PTEVKIQEMT KLGHELMLCA
121PDDQELLKGC ACAQKQLHFM DQLLDTIRSL TIGCSSCSSL MEHFEDTREK NEALLGELFS
181SPHLQMLLNP ECDPWPLDMQ PLLNKQSDDW QWASASAKSE EEEKLAELAR QLQESAAKLH
241ALRTEYFAQH EQGAAAGAAD ISTLDQKLRL VTSDFHQLIL AFLQVYDDEL GECCQRPGPD
301LHPCGPIIQA THQNLTSYSQ LLQVVMAVAD TSAKAVETVK KQQGEQICWG GSSSVMSLAT
361KMNELMEK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

368 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.