Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HAUS6 / FAM29A Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G112318-100
Catalog Number: LS-G112318
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM29A(HAUS6) (NM_017645), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Dgt6 | Dim gamma-tubulin homolog | FAM29A | KIAA1574 | HAUS6 | RP11-296P7.3
- Origin Species
- Human
- NCBI GenBank
- NM_017645
- SwissProt
- Q7Z4H7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 108.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSSASVTAFE KEHLWMYLQA LGFEPGPATI ACGKIVSHTH LGVNMFDKLN RDAFHIISYF61LFQVLDQSLT KEVFKFCWPP FDQKSDTEFR KHCCEWIKRI SGECGSSFPQ VVGSLFLSPG121GPKFIHLMYH FARFVAMKYI KSNSKNSSHH FVETFNIKPQ DLHKCIARCH FARSRFLQIL181QRQDCVTQKY QENAQLSVKQ VRNLRSECIG LENQIKKMEP YDDHSNMEEK IQKVRSLWAS241VNETLMFLEK EREVVSSVLS LVNQYALDGT NVAINIPRLL LDKIEKQMFQ LHIGNVYEAG301KLNLLTVIQL LNEVLKVMKY ERCQADQARL TVDLHYLEKE TKFQKERLSD LKHMRYRIKD361DLTTIRHSVV EKQGEWHKKW KEFLGLSPFS LIKGWTPSVD LLPPMSPLSF DPASEEVYAK421SILCQYPASL PDAHKQHNQE NGCRGDSDTL GALHDLANSP ASFLSQSVSS SDRNSVTVLE481KDTKMGTPKE KNEAISKKIP EFEVENSPLS DVAKNTESSA FGGSLPAKKS DPFQKEQDHL541VEEVARAVLS DSPQLSEGKE IKLEELIDSL GSNPFLTRNQ IPRTPENLIT EIRSSWRKAI601EMEENRTKEP IQMDVEHREV LPESLPVLHN QREFSMADFL LETTVSDFGQ SHLTEEKVIS661DCECVPQKHV LTSHIDEPPT QNQSDLLNKK VICKQDLECL AFTKLSETSR METFSPAVGN721RIDVMGGSEE EFMKILDHLE VSCNKPSTNK TMLWNSFQIS IGISSKSFKD NDFGILHETL781PEEVGHLSFN SSSSSEANFK LEPNSPMHGG TLLEDVVGGR QTTPESDFNL QALRSRYEAL841KKSLSKKREE SYLSNSQTPE RHKPELSPTP QNVQTDDTLN FLDTCDLHTE HIKPSLRTSI901GERKRSLSPL IKFSPVEQRL RTTIACSLGE LPNLKEEDIL NKSLDAKEPP SDLTRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
955 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.