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HAUS5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HAUS5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all HAUS5 antibodies →
$725.00each
Catalog Number: LS-G113307-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HAUS5 (NM_015302), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0841 | HAUS5 | Dgt5
Origin Species
Human
NCBI GenBank
AB020648
SwissProt
O94927

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
71.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MELAQEAREL GCWAVEEMGV PVAARAPEST LRRLCLGQGA DIWAYILQHV HSQRTVKKIR
61GNLLWYGHQD SPQVRRKLEL EAAVTRLRAE IQELDQSLEL MERDTEAQDT AMEQARQHTQ
121DTQRRALLLR AQAGAMRRQQ HTLRDPMQRL QNQLRRLQDM ERKAKVDVTF GSLTSAALGL
181EPVVLRDVRT ACTLRAQFLQ NLLLPQAKRG SLPTPHDDHF GTSYQQWLSS VETLLTNHPP
241GHVLAALEHL AAEREAEIRS LCSGDGLGDT EISRPQAPDQ SDSSQTLPSM VHLIQEGWRT
301VGVLVSQRST LLKERQVLTQ RLQGLVEEVE RRVLGSSERQ VLILGLRRCC LWTELKALHD
361QSQELQDAAG HRQLLLRELQ AKQQRILHWR QLVEETQEQV RLLIKGNSAS KTRLCRSPGE
421VLALVQRKVV PTFEAVAPQS RELLRCLEEE VRHLPHILLG TLLRHRPGEL KPLPTVLPSI
481HQLHPASPRG SSFIALSHKL GLPPGKASEL LLPAAASLRQ DLLLLQDQRS LWCWDLLHMK
541TSLPPGLPTQ ELLQIQASQE KQQKENLGQA LKRLEKLLKQ ALERIPELQG IVGDWWEQPG
601QAALSEELCQ GLSLPQWRLR WVQAQGALQK LCS
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

633 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.