Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HAUS3 / C4orf15 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G109945-100
Catalog Number: LS-G109945
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HAUS3 (NM_024511), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C4orf15 | HAUS3 | Dgt3 | IT1
- Origin Species
- Human
- NCBI GenBank
- AF040964
- SwissProt
- Q68CZ6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 69.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASVTSHKSL RLNAKEEEAT KKLKQSQGIL NAMITKISNE LQALTDEVTQ LMMFFRHSNL61GQGTNPLVFL SQFSLEKYLS QEEQSTAALT LYTKKQFFQG IHEVVESSNE DNFQLLDIQT121PSICDNQEIL EERRLEMARL QLAYICAQHQ LIHLKASNSS MKSSIKWAEE SLHSLTSKAV181DKENLDAKIS SLTSEIMKLE KEVTQIKDRS LPAVVRENAQ LLNMPVVKGD FDLQIAKQDY241YTARQELVLN QLIKQKASFE LLQLSYEIEL RKHRDIYRQL ENLVQELSQS NMMLYKQLEM301LTDPSVSQQI NPRNTIDTKD YSTHRLYQVL EGENKKKELF LTHGNLEEVA EKLKQNISLV361QDQLAVSAQE HSFFLSKRNK DVDMLCDTLY QGGNQLLLSD QELTEQFHKV ESQLNKLNHL421LTDILADVKT KRKTLANNKL HQMEREFYVY FLKDEDYLKD IVENLETQSK IKAVSLEDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
478 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.