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HAS3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HAS3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all HAS3 antibodies →
$725.00each
Catalog Number: LS-G116039-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HAS3 (NM_005329), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Hyaluronan synthase 3 | Hyaluronate synthase 3 | HA synthase 3 | HAS3 | Hyaluronic acid synthase 3
Origin Species
Human
NCBI GenBank
NM_005329
SwissProt
O00219

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
62.8 kda
Protein Type
Lysate
Amino Acid Sequence
1MPVQLTTALR VVGTSLFALA VLGGILAAYV TGYQFIHTEK HYLSFGLYGA ILGLHLLIQS
61LFAFLEHRRM RRAGQALKLP SPRRGSVALC IAAYQEDPDY LRKCLRSAQR ISFPDLKVVM
121VVDGNRQEDA YMLDIFHEVL GGTEQAGFFV WRSNFHEAGE GETEASLQEG MDRVRDVVRA
181STFSCIMQKW GGKREVMYTA FKALGDSVDY IQVCDSDTVL DPACTIEMLR VLEEDPQVGG
241VGGDVQILNK YDSWISFLSS VRYWMAFNVE RACQSYFGCV QCISGPLGMY RNSLLQQFLE
301DWYHQKFLGS KCSFGDDRHL TNRVLSLGYR TKYTARSKCL TETPTKYLRW LNQQTRWSKS
361YFREWLYNSL WFHKHHLWMT YESVVTGFFP FFLIATVIQL FYRGRIWNIL LFLLTVQLVG
421IIKATYACFL RGNAEMIFMS LYSLLYMSSL LPAKIFAIAT INKSGWGTSG RKTIVVNFIG
481LIPVSIWVAV LLGGLAYTAY CQDLFSETEL AFLVSGAILY GCYWVALLML YLAIIARRCG
541KKPEQYSLAF AEV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

553 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.