Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Harmonin / USH1C Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G105736-100
Catalog Number: LS-G105736
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human USH1C (NM_153676), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AIE-75 | Antigen NY-CO-38/NY-CO-37 | AIE75 | DFNB18 | DFNB18A | Harmonin | PDZ-45 | NY-CO-38 | USH1C | Usher syndrome type-1C protein | Ush1cpst | NY-CO-37 | PDZ-73 | PDZ73 | Protein PDZ-73
- Origin Species
- Human
- NCBI GenBank
- NM_005709
- SwissProt
- Q9Y6N9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 101.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDRKVAREFR HKVDFLIEND AEKDYLYDVL RMYHQTMDVA VLVGDLKLVI NEPSRLPLFD61AIRPLIPLKH QVEYDQLTPR RSRKLKEVRL DRLHPEGLGL SVRGGLEFGC GLFISHLIKG121GQADSVGLQV GDEIVRINGY SISSCTHEEV INLIRTKKTV SIKVRHIGLI PVKSSPDEPL181TWQYVDQFVS ESGGVRGSLG SPGNRENKEK KVFISLVGSR GLGCSISSGP IQKPGIFISH241VKPGSLSAEV GLEIGDQIVE VNGVDFSNLD HKEAVNVLKS SRSLTISIVA AAGRELFMTD301RERLAEARQR ELQRQELLMQ KRLAMESNKI LQEQQEMERQ RRKEIAQKAA EENERYRKEM361EQIVEEEEKF KKQWEEDWGS KEQLLLPKTI TAEVHPVPLR KPKSFGWFYR YDGKFPTIRK421KGKDKKKAKY GSLQDLRKNK KELEFEQKLY KEKEEMLEKE KQLKINRLAQ EVSETEREDL481EESEKIQYWV ERLCQTRLEQ ISSADNEISE MTTGPPPPPP SVSPLAPPLR RFAGGLHLHT541TDLDDIPLDM FYYPPKTPSA LPVMPHPPPS NPPHKVPAPP VLPLSGHVSA SSSPWVQRTP601PPIPIPPPPS VPTQDLTPTR PLPSALEEAL SNHPFRTGDT GNPVEDWEAK NHSGKPTNSP661VPEQSFPPTP KTFCPSPQPP RGPGVSTISK PVMVHQEPNF IYRPAVKSEV LPQEMLKRMV721VYQTAFRQDF RKYEEGFDPY SMFTPEQIMG KDVRLLRIKK EGSLDLALEG GVDSPIGKVV781VSAVYERGAA ERHGGIVKGD EIMAINGKIV TDYTLAEAEA ALQKAWNQGG DWIDLVVAVC841PPKEYDDELA SLPSSVAESP QPVRKLLEDR AAVHRHGFLL QLEPTDLLLK SKRGNQIHRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
899 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.