Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Hairless / HR Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G111745-100
Catalog Number: LS-G111745
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Hairless(HR) (NM_018411), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ALUNC | Hairless | Hairless homolog (mouse) | HR | HSA277165 | MUHH | Protein hairless | AU | Hair growth associated | Hairless (mouse) homolog | MUHH1
- Origin Species
- Human
- NCBI GenBank
- NM_018411
- SwissProt
- O43593
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 121.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MESTPSFLKG TPTWEKTAPE NGIVRQEPGS PPRDGLHHGP LCLGEPAPFW RGVLSTPDSW61LPPGFPQGPK DMLPLVEGEG PQNGERKVNW LGSKEGLRWK EAMLTHPLAF CGPACPPRCG121PLMPEHSGGH LKSDPVAFRP WHCPFLLETK ILERAPFWVP TCLPPYLVSG LPPEHPCDWP181LTPHPWVYSG GQPKVPSAFS LGSKGFYYKD PSIPRLAKEP LAAAEPGLFG LNSGGHLQRA241GEAERPSLHQ RDGEMGAGRQ QNPCPLFLGQ PDTVPWTSWP ACPPGLVHTL GNVWAGPGDG301NLGYQLGPPA TPRCPSPEPP VTQRGCCSSY PPTKGGGLGP CGKCQEGLEG GASGASEPSE361EVNKASGPRA CPPSHHTKLK KTWLTRHSEQ FECPRGCPEV EERPVARLRA LKRAGSPEVQ421GAMGSPAPKR PPDPFPGTAE QGAGGWQEVR DTSIGNKDVD SGQHDEQKGP QDGQASLQDP481GLQDIPCLAL PAKLAQCQSC AQAAGEGGGH ACHSQQVRRS PLGGELQQEE DTATNSSSEE541GPGSGPDSRL STGLAKHLLS GLGDRLCRLL RREREALAWA QREGQGPAVT EDSPGIPRCC601SRCHHGLFNT HWRCPRCSHR LCVACGRVAG TGRAREKAGF QEQSAEECTQ EAGHAACSLM661LTQFVSSQAL AELSTAMHQV WVKFDIRGHC PCQADARVWA PGDAGQQKES TQKTPPTPQP721SCNGDTHRTK SIKEETPDSA ETPAEDRAGR GPLPCPSLCE LLASTAVKLC LGHERIHMAF781APVTPALPSD DRITNILDSI IAQVVERKIQ EKALGPGLRA GPGLRKGLGL PLSPVRPRLP841PPGALLWLQE PQPCPRRGFH LFQEHWRQGQ PVLVSGIQRT LQGNLWGTEA LGALGGQVQA901LSPLGPPQPS SLGSTTFWEG FSWPELRPKS DEGSVLLLHR ALGDEDTSRV ENLAASLPLP961EYCALHGKLN LASYLPPGLA LRPLEPQLWA AYGVSPHRGH LGTKNLCVEV ADLVSILVHA1021DTPLPAWHRA QKDFLSGLDG EGLWSPGSQV STVWHVFRAQ DAQRIRRFLQ MVQGLVSTVS1081VTQHFLSPET SALSAQLCHQ GPSLPPDCHL LYAQMDWAVF QAVKVAVGTL QEAKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1134 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.